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Vahabi S, Fekrazad R, Ayremlou S, et al. The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: an in vitro study. J Dent (Tehran). 2011;8(2):48-54
Vahabi, S., Fekrazad, R., Ayremlou, S., Taheri, S., & Zangeneh, N. (2011). The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: an in vitro study. Journal of dentistry (Tehran, Iran), 8(2), 48-54.
Vahabi, S, et al. "The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: an in vitro study." Journal of dentistry (Tehran, Iran) vol. 8,2 (2011): 48-54.
Vahabi S, Fekrazad R, Ayremlou S, Taheri S, Zangeneh N. The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: an in vitro study. J Dent (Tehran). 2011;8(2):48-54. Epub 2011 Jun 30. PMID: 21998808; PMCID: PMC3184734.
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J Dent (Tehran). 2011;8(2):48-54. Epub 2011 Jun 30.

The effect of antimicrobial photodynamic therapy with radachlorin and toluidine blue on streptococcus mutans: an in vitro study.

Journal of dentistry (Tehran, Iran)

S Vahabi, R Fekrazad, S Ayremlou, S Taheri, N Zangeneh

Affiliations

  1. Assistant Professor, Department of Periodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 21998808 PMCID: PMC3184734

Abstract

OBJECTIVES: Dental caries and periodontal diseases are caused by infection of teeth and supporting tissues due to complex aggregate of bacteria known as biofilm, firstly colonized by streptococci. The main purpose of this in vitro study was to evaluate the antimicrobial effects of toluidine blue O (TBO) and Radachlorin® in combination with a diode laser on the viability of Streptococcus mutans.

MATERIALS AND METHODS: Bacterial suspensions of Streptococcus mutans were exposed to either 0.1% TBO associated with (20 mW, 633 nm diode laser, continuous mode, 150 s) or 0.1% Radachlorin® and laser irradiation (100 mW, 662 nm diode laser, continuous mode, 120 s). Those in control groups were subjected to laser irradiation alone or TBO/Radachlorin® alone or received neither TBO/Radachlorin® nor laser exposure. The suspensions were then spread over specific agar plates and incubated aerobically at 37°C. Finally, the bactericidal effects were evaluated based on colony formation.

RESULTS: Potential bacterial cell killing was only observed following photosensitization with TBO and 3 j/cm(2) laser exposure (p<0.05), whereas Radachlorin® showed significant reduction in dark condition compared to laser exposure (p<0.05).

CONCLUSION: TBO-mediated photodynamic therapy seems to be more efficient than Radachlorin® in significantly reducing the viability of Streptococcus mutans in vitro.

Keywords: Antimicrobial Photodynamic Therapy (APDT); Diode Laser; Radachlorin®; Streptococcus Mutans; Toluidine Blue

References

  1. Appl Environ Microbiol. 2003 Nov;69(11):6393-8 - PubMed
  2. J Calif Dent Assoc. 2000 Mar;28(3):185-93 - PubMed
  3. Photochem Photobiol. 1997 Jun;65(6):1026-31 - PubMed
  4. Lasers Med Sci. 2009 Mar;24(2):162-6 - PubMed
  5. J Periodontol. 2004 Oct;75(10):1343-9 - PubMed
  6. Curr Drug Metab. 2009 Jan;10(1):90-4 - PubMed
  7. J Clin Laser Med Surg. 2002 Feb;20(1):3-7 - PubMed
  8. Zh Mikrobiol Epidemiol Immunobiol. 2005 May-Jun;(3):22-7 - PubMed
  9. Trends Microbiol. 1994 Jun;2(6):209-12 - PubMed
  10. Pol J Microbiol. 2005;54(4):305-10 - PubMed
  11. Lasers Med Sci. 2003;18(1):51-5 - PubMed
  12. Science. 1999 May 21;284(5418):1318-22 - PubMed
  13. J Bacteriol. 2001 Jun;183(12):3770-83 - PubMed
  14. Antimicrob Agents Chemother. 2000 Dec;44(12):3364-7 - PubMed
  15. J Antimicrob Chemother. 1998 Jul;42(1):13-28 - PubMed
  16. J Periodontol. 2010 Jul;81(7):975-81 - PubMed
  17. Sci Prog. 2001;84(Pt 3):235-54 - PubMed
  18. Microbiology (Reading). 2005 Feb;151(Pt 2):625-631 - PubMed
  19. Photodiagnosis Photodyn Ther. 2004 May;1(1):27-42 - PubMed
  20. Photochem Photobiol. 1998 Sep;68(3):370-6 - PubMed
  21. Photomed Laser Surg. 2006 Feb;24(1):59-63 - PubMed
  22. Photochem Photobiol Sci. 2004 May;3(5):412-8 - PubMed
  23. J Photochem Photobiol B. 1990 May;5(3-4):281-93 - PubMed
  24. Caries Res. 2004 May-Jun;38(3):230-5 - PubMed
  25. Int Dent J. 2000 Jun;50(3):129-39 - PubMed
  26. Eur J Oral Sci. 2007 Feb;115(1):77-80 - PubMed
  27. Antimicrob Agents Chemother. 1998 Oct;42(10):2595-601 - PubMed
  28. J Periodontal Res. 2003 Aug;38(4):428-35 - PubMed
  29. J Photochem Photobiol B. 1999 May;50(1):1-7 - PubMed
  30. Microbiol Res. 2008;163(1):1-12 - PubMed
  31. Periodontol 2000. 2009;51:109-40 - PubMed
  32. Nanotechnology. 2008 Nov 5;19(44):445102 - PubMed

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