Contemp Clin Dent. 2010 Jul;1(3):164-7. doi: 10.4103/0976-237X.72784.
In vitro antimicrobial activity of root canal sealers and calcium hydroxide paste.
Contemporary clinical dentistry
Alessandro L Cavalcanti, Francisco I R Limeira, Eveline A L S Sales, Ana A G Oliveira, Dened M B Lima, Ricardo D Castro
Affiliations
Affiliations
- School of Dentistry, State University of Paraiba, Campina Grande, Brazil.
PMID: 22114408
PMCID: PMC3220103 DOI: 10.4103/0976-237X.72784
Abstract
AIM: To evaluate the in vitro antimicrobial activity of different root canal sealers and calcium hydroxide (CH) paste.
MATERIALS AND METHODS: The sample was composed of two sealers (Fill Canal(®) and Sealer 26(®)), one CH cement (Hydro C(®)), and a CH paste. The agar diffusion test was performed in Petri dishes inoculated with the following microorganisms: Streptococcus salivarius, Streptococcus oralis, Streptococcus mitis, Lactobacillus casei, Streptococcus mutans, Candida albicans, Candida krusei, and Candida tropicalis. The diameters of the zones of microbial growth inhibition were measured after 24 h. The tests were performed in triplicate. Data were analyzed statistically by ANOVA and Tukey's test at 5% significance level.
RESULTS: Fill Canal(®) exhibited the largest mean zone of microbial growth inhibition against the Candida species and differed significantly from the other groups (P < 0.001). When inhibition was observed against S. mitis and S. oralis, the CH paste presented a larger mean zone of microbial growth inhibition than those of the other materials (P < 0.05). Regarding the inhibition of S. mutans, a statistically significant difference was observed only between the CH paste and Hydro C(®) (P < 0.05); the paste produced the largest mean zone of microbial growth inhibition against this microorganism. Regarding the inhibition of S. salivarius, Fill Canal(®) presented smaller mean zone of microbial growth inhibition than Sealer 26(®) and CH paste (P < 0.05).
CONCLUSION: All the materials presented zones of microbial growth inhibition against all the test bacteria. Fill Canal(®) presented the largest mean zone of inhibition against the Candida species. For the Streptococcus cultures, none of the sealers presented inhibition superior to that of the CH paste.
Keywords: Candida; microbiological techniques; root canal filling materials
References
- J Endod. 1980 Jun;6(6):594-7 - PubMed
- Braz Dent J. 1999;10(2):63-72 - PubMed
- Oral Surg Oral Med Oral Pathol. 1994 Nov;78(5):634-45 - PubMed
- Braz Dent J. 2008;19(4):301-5 - PubMed
- J Endod. 2000 Jul;26(7):391-4 - PubMed
- Int Endod J. 1995 Nov;28(6):285-9 - PubMed
- J Contemp Dent Pract. 2008 Jul 01;9(5):60-7 - PubMed
- Clin Oral Investig. 2001 Dec;5(4):236-9 - PubMed
- Oral Microbiol Immunol. 2004 Apr;19(2):71-6 - PubMed
- J Endod. 2000 May;26(5):274-7 - PubMed
- J Appl Oral Sci. 2003 Mar;11(1):55-9 - PubMed
- J Am Dent Assoc. 2007 Sep;138(9):1228-32 - PubMed
- J Oral Sci. 2007 Mar;49(1):41-5 - PubMed
- Braz Oral Res. 2006 Oct-Dec;20(4):303-6 - PubMed
- Braz Dent J. 2004;15(1):30-5 - PubMed
- J Appl Oral Sci. 2003 Dec;11(4):269-82 - PubMed
- J Endod. 2002 May;28(5):365-6 - PubMed
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