Display options
Cite
Fajardo V, González I, López-Calleja I, et al. Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene. Meat Sci. 2007;76(2):234-40doi: 10.1016/j.meatsci.2006.11.004.
Fajardo, V., González, I., López-Calleja, I., Martín, I., Rojas, M., Hernández, P. E., García, T., & Martín, R. (2007). Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene. Meat science, 76(2), 234-40. https://doi.org/10.1016/j.meatsci.2006.11.004
Fajardo, V, et al. "Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene." Meat science vol. 76,2 (2007): 234-40. doi: https://doi.org/10.1016/j.meatsci.2006.11.004
Fajardo V, González I, López-Calleja I, Martín I, Rojas M, Hernández PE, García T, Martín R. Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene. Meat Sci. 2007 Jun;76(2):234-40. doi: 10.1016/j.meatsci.2006.11.004. Epub 2007 Jan 03. PMID: 22064291.
Copy Download .nbib Format: NLM
Share it on

Meat Sci. 2007 Jun;76(2):234-40. doi: 10.1016/j.meatsci.2006.11.004. Epub 2007 Jan 03.

Identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) using polymerase chain reaction targeting specific sequences from the mitochondrial 12S rRNA gene.

Meat science

V Fajardo, I González, I López-Calleja, I Martín, M Rojas, P E Hernández, T García, Rosario Martín

Affiliations

  1. Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain.

PMID: 22064291 DOI: 10.1016/j.meatsci.2006.11.004

Abstract

Polymerase chain reaction (PCR) based on oligonucleotide primers targeting the mitochondrial 12S rRNA gene was applied to the specific identification of meats from red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus). The use of a common reverse primer, together with forward specific primers for red deer, fallow deer, and roe deer, allowed the selective amplification of the desired cervid sequences. The specificity of each primer pair was verified by PCR analysis of DNA from various game and domestic meats. The assay can be useful for the accurate identification of meats from cervid species, avoiding mislabeling or fraudulent species substitution in meat products.

Publication Types