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Dupont JA, Snoussi K. Effects of magnesium ions on two EMCV IRES key activity fragments. J Biol Phys. 2010;37(1):61-8doi: 10.1007/s10867-010-9198-0.
Dupont, J. A., & Snoussi, K. (2011). Effects of magnesium ions on two EMCV IRES key activity fragments. Journal of biological physics, 37(1), 61-8. https://doi.org/10.1007/s10867-010-9198-0
Dupont, Julien A, and Snoussi, Karim. "Effects of magnesium ions on two EMCV IRES key activity fragments." Journal of biological physics vol. 37,1 (2011): 61-8. doi: https://doi.org/10.1007/s10867-010-9198-0
Dupont JA, Snoussi K. Effects of magnesium ions on two EMCV IRES key activity fragments. J Biol Phys. 2011 Jan;37(1):61-8. doi: 10.1007/s10867-010-9198-0. Epub 2010 Aug 21. PMID: 22210961; PMCID: PMC3006467.
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J Biol Phys. 2011 Jan;37(1):61-8. doi: 10.1007/s10867-010-9198-0. Epub 2010 Aug 21.

Effects of magnesium ions on two EMCV IRES key activity fragments.

Journal of biological physics

Julien A Dupont, Karim Snoussi

PMID: 22210961 PMCID: PMC3006467 DOI: 10.1007/s10867-010-9198-0

Abstract

Using NMR magnetization transfer from water and ammonia-catalyzed exchange of the imino protons, changes have been monitored in base-pair kinetics induced by Mg(2 + ) in two key activity fragments r(CACCUGGCGACAGGUG) and r(GGCCAAAAGCC) of the encephalomyocarditis virus internal ribosome entry site. For r(CACCUGGCGACAGGUG), the addition of Mg(2 + ) reveals two types of base-pairs: r(U(545)·A) and r(G(546)·C), in the first category, have lifetimes only slightly higher in the presence of Mg(2 + ), whereas their dissociation constants are substantially reduced. This behavior has been termed proximal. The base-pairs r(G(553)·C) and r(G(554)·C), in the second category, have lifetimes substantially higher in the presence of Mg(2 + ), whereas their dissociation constants remain almost constant. This behavior has been termed distal. Mg(2 + ) has a specific effect on r(CACCUGGCGACAGGUG), the magnitude of which is progressively modulated from the proximal region of the 16-mer towards its distal region. For r(GGCCAAAAGCC), an intermediate behavior is found for base-pairs r(G(565)·C) and r(G(572)·C). Their lifetimes are slightly higher in the presence of Mg(2 + ) and their dissociation constants are significantly lower, a behavior resembling that of the 16-mer proximal region. These results indicate that Mg(2 + ) diffusively moves around r(GGCCAAAAGCC).

Keywords: Base-pair kinetics; EMCV IRES; GNRA tetraloop; Imino proton exchange; Mg2 + ; NMR; RAAA hairpin motif

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