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Schoorl M, Bartels PC, Gritters M, et al. Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject. Hematol Rep. 2011;3(2):e15doi: 10.4081/hr.2011.e15.
Schoorl, M., Bartels, P. C., Gritters, M., Fluitsma, D., Musters, R., & Nubé, M. J. (2011). Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject. Hematology reports, 3(2), e15. https://doi.org/10.4081/hr.2011.e15
Schoorl, Marianne, et al. "Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject." Hematology reports vol. 3,2 (2011): e15. doi: https://doi.org/10.4081/hr.2011.e15
Schoorl M, Bartels PC, Gritters M, Fluitsma D, Musters R, Nubé MJ. Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject. Hematol Rep. 2011 Aug 31;3(2):e15. doi: 10.4081/hr.2011.e15. Epub 2011 Oct 19. PMID: 22184536; PMCID: PMC3238486.
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Hematol Rep. 2011 Aug 31;3(2):e15. doi: 10.4081/hr.2011.e15. Epub 2011 Oct 19.

Electron microscopic observation in case of platelet activation in a chronic haemodialysis subject.

Hematology reports

Marianne Schoorl, Piet C M Bartels, Mareille Gritters, Donna Fluitsma, René Musters, Menso J Nubé

Affiliations

  1. Department of Clinical Chemistry, Haematology and Immunology, Medical Center Alkmaar, Alkmaar;

PMID: 22184536 PMCID: PMC3238486 DOI: 10.4081/hr.2011.e15

Abstract

During haemodialysis (HD), platelets (PLTs) are activated and release granule contents. As HD treatment occurs three times a week, it has been demonstrated that PLTs are exhausted due to the repetitive character of the treatment. To identify PLT depletion morphologically, PLT evaluation was performed by light microscopy and electron microscopy (EM) in a chronic HD subject and a healthy reference subject. Blood samples were taken before the start of HD treatment for measurement of PLT count, PLT volume and size parameters. Blood smears were screened by light microscopy for qualitative evaluation of PLT granule containing cytoplasm, as indicated by its staining density. Morphological PLT parameters of surface area and size of dense bodies were assessed by EM. Data were compared with results of a group of 20 chronic HD subjects and a group of 20 healthy reference subjects. With respect to the percentage of PLTs with appropriate staining density (>75%), light microscopic evaluation showed that this value (9%) was within the range of a group of chronic HD subjects, but considerably below the reference range (70%). EM evaluation revealed an average PLT surface area and dense bodies area of respectively 42% and 31%, if the healthy reference subject was set on 100%. PLTs from a chronic HD subject are considerably smaller and substantially less granular than PLTs from a healthy reference subject. These findings support the hypothesis of PLT depletion in chronic HD subjects due to frequent PLT activation and/or increased urea concentrations.

Keywords: electron microscopy; haemodialysis.; platelet activation; platelet degranulation

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