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Silva ON, Mulder KC, Barbosa AE, et al. Exploring the pharmacological potential of promiscuous host-defense peptides: from natural screenings to biotechnological applications. Front Microbiol. 2011;2:232doi: 10.3389/fmicb.2011.00232.
Silva, O. N., Mulder, K. C., Barbosa, A. E., Otero-Gonzalez, A. J., Lopez-Abarrategui, C., Rezende, T. M., Dias, S. C., & Franco, O. L. (2011). Exploring the pharmacological potential of promiscuous host-defense peptides: from natural screenings to biotechnological applications. Frontiers in microbiology, 2232. https://doi.org/10.3389/fmicb.2011.00232
Silva, Osmar N, et al. "Exploring the pharmacological potential of promiscuous host-defense peptides: from natural screenings to biotechnological applications." Frontiers in microbiology vol. 2 (2011): 232. doi: https://doi.org/10.3389/fmicb.2011.00232
Silva ON, Mulder KC, Barbosa AE, Otero-Gonzalez AJ, Lopez-Abarrategui C, Rezende TM, Dias SC, Franco OL. Exploring the pharmacological potential of promiscuous host-defense peptides: from natural screenings to biotechnological applications. Front Microbiol. 2011 Nov 22;2:232. doi: 10.3389/fmicb.2011.00232. eCollection 2011. PMID: 22125552; PMCID: PMC3222093.
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Front Microbiol. 2011 Nov 22;2:232. doi: 10.3389/fmicb.2011.00232. eCollection 2011.

Exploring the pharmacological potential of promiscuous host-defense peptides: from natural screenings to biotechnological applications.

Frontiers in microbiology

Osmar N Silva, Kelly C L Mulder, Aulus E A D Barbosa, Anselmo J Otero-Gonzalez, Carlos Lopez-Abarrategui, Taia M B Rezende, Simoni C Dias, Octávio L Franco

Affiliations

  1. Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Centro de Análises Protômicas e Bioquímicas, Universidade Católica de Brasília Brasília, Brazil.

PMID: 22125552 PMCID: PMC3222093 DOI: 10.3389/fmicb.2011.00232

Abstract

In the last few years, the number of bacteria with enhanced resistance to conventional antibiotics has dramatically increased. Most of such bacteria belong to regular microbial flora, becoming a real challenge, especially for immune-depressed patients. Since the treatment is sometimes extremely expensive, and in some circumstances completely inefficient for the most severe cases, researchers are still determined to discover novel compounds. Among them, host-defense peptides (HDPs) have been found as the first natural barrier against microorganisms in nearly all living groups. This molecular class has been gaining attention every day for multiple reasons. For decades, it was believed that these defense peptides had been involved only with the permeation of the lipid bilayer in pathogen membranes, their main target. Currently, it is known that these peptides can bind to numerous targets, as well as lipids including proteins and carbohydrates, from the surface to deep within the cell. Moreover, by using in vivo models, it was shown that HDPs could act both in pathogens and cognate hosts, improving immunological functions as well as acting through multiple pathways to control infections. This review focuses on structural and functional properties of HDP peptides and the additional strategies used to select them. Furthermore, strategies to avoid problems in large-scale manufacture by using molecular and biochemical techniques will also be explored. In summary, this review intends to construct a bridge between academic research and pharmaceutical industry, providing novel insights into the utilization of HDPs against resistant bacterial strains that cause infections in humans.

Keywords: antimicrobials; host-defense peptides; innate immunity; microbial infections

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