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Lestienne PP. Priming DNA replication from triple helix oligonucleotides: possible threestranded DNA in DNA polymerases. Mol Biol Int. 2011;2011:562849doi: 10.4061/2011/562849.
Lestienne, P. P. (2011). Priming DNA replication from triple helix oligonucleotides: possible threestranded DNA in DNA polymerases. Molecular biology international, 2011562849. https://doi.org/10.4061/2011/562849
Lestienne, Patrick P. "Priming DNA replication from triple helix oligonucleotides: possible threestranded DNA in DNA polymerases." Molecular biology international vol. 2011 (2011): 562849. doi: https://doi.org/10.4061/2011/562849
Lestienne PP. Priming DNA replication from triple helix oligonucleotides: possible threestranded DNA in DNA polymerases. Mol Biol Int. 2011;2011:562849. doi: 10.4061/2011/562849. Epub 2011 Sep 14. PMID: 22229092; PMCID: PMC3200174.
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Mol Biol Int. 2011;2011:562849. doi: 10.4061/2011/562849. Epub 2011 Sep 14.

Priming DNA replication from triple helix oligonucleotides: possible threestranded DNA in DNA polymerases.

Molecular biology international

Patrick P Lestienne

Affiliations

  1. U 1053 INSERM, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux, France.

PMID: 22229092 PMCID: PMC3200174 DOI: 10.4061/2011/562849

Abstract

Triplex associate with a duplex DNA presenting the same polypurine or polypyrimidine-rich sequence in an antiparallel orientation. So far, triplex forming oligonucleotides (TFOs) are known to inhibit transcription, replication, and to induce mutations. A new property of TFO is reviewed here upon analysis of DNA breakpoint yielding DNA rearrangements; the synthesized sequence of the first direct repeat displays a skewed polypurine- rich sequence. This synthesized sequence can bind the second homologous duplex sequence through the formation of a triple helix, which is able to prime further DNA replication. In these case, the d(G)-rich Triple Helix Primers (THP) bind the homologous strand in a parallel manner, possibly via a RecA-like mechanism. This novel property is shared by all tested DNA polymerases: phage, retrovirus, bacteria, and human. These features may account for illegitimate initiation of replication upon single-strand breakage and annealing to a homologous sequence where priming may occur. Our experiments suggest that DNA polymerases can bind three instead of two polynucleotide strands in their catalytic centre.

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