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Vertino A, Ayyadevara S, Thaden JJ, et al. A narrow quantitative trait locus in C. elegans coordinately affects longevity, thermotolerance, and resistance to paraquat. Front Genet. 2011;2:63doi: 10.3389/fgene.2011.00063.
Vertino, A., Ayyadevara, S., Thaden, J. J., & Shmookler Reis, R. J. (2011). A narrow quantitative trait locus in C. elegans coordinately affects longevity, thermotolerance, and resistance to paraquat. Frontiers in genetics, 263. https://doi.org/10.3389/fgene.2011.00063
Vertino, Anthony, et al. "A narrow quantitative trait locus in C. elegans coordinately affects longevity, thermotolerance, and resistance to paraquat." Frontiers in genetics vol. 2 (2011): 63. doi: https://doi.org/10.3389/fgene.2011.00063
Vertino A, Ayyadevara S, Thaden JJ, Shmookler Reis RJ. A narrow quantitative trait locus in C. elegans coordinately affects longevity, thermotolerance, and resistance to paraquat. Front Genet. 2011 Sep 27;2:63. doi: 10.3389/fgene.2011.00063. eCollection 2011. PMID: 22303358; PMCID: PMC3268616.
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Front Genet. 2011 Sep 27;2:63. doi: 10.3389/fgene.2011.00063. eCollection 2011.

A narrow quantitative trait locus in C. elegans coordinately affects longevity, thermotolerance, and resistance to paraquat.

Frontiers in genetics

Anthony Vertino, Srinivas Ayyadevara, John J Thaden, Robert J Shmookler Reis

Affiliations

  1. Veterans Affairs Medical Center, Central Arkansas Veterans Health Care System Little Rock, and University of Arkansas for Medical Sciences, Little Rock, AR, USA.

PMID: 22303358 PMCID: PMC3268616 DOI: 10.3389/fgene.2011.00063

Abstract

By linkage mapping of quantitative trait loci, we previously identified at least 11 natural genetic variants that significantly modulate Caenorhabditis elegans life-span (LS), many of which would have eluded discovery by knock-down or mutation screens. A region on chromosome IV between markers stP13 and stP35 had striking effects on longevity in three inter-strain crosses (each P < 10(-9)). In order to define the limits of that interval, we have now constructed two independent lines by marker-based selection during 20 backcross generations, isolating the stP13-stP35 interval from strain Bergerac-BO in a CL2a background. These congenic lines differed significantly from CL2a in LS, assayed in two environments (each P < 0.001). We then screened for exchange of flanking markers to isolate recombinants that partition this region, because fine-mapping the boundaries for overlapping heteroallelic spans can greatly narrow the implicated interval. Recombinants carrying the CL2a allele at stP35 were consistently long-lived compared to those retaining the Bergerac-BO allele (P < 0.001), and more resistant to temperature elevation and paraquat (each ∼1.7-fold, P < 0.0001), but gained little protection from ultraviolet or peroxide stresses. Two rounds of recombinant screening, followed by fine-mapping of break-points and survival testing, narrowed the interval to 0.18 Mb (13.35-13.53 Mb) containing 26 putative genes and six small-nuclear RNAs - a manageable number of targets for functional assessment.

Keywords: life-span; linkage mapping; longevity; oxidative stress; recombinant-congenic lines; thermotolerance; ultraviolet irradiation, C. elegans

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