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Takaoka E, Sonobe H, Akimaru K, et al. Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line. Am J Cancer Res. 2012;2(2):141-52
Takaoka, E., Sonobe, H., Akimaru, K., Sakamoto, S., Shuin, T., Daibata, M., Taguchi, T., & Tominaga, A. (2012). Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line. American journal of cancer research, 2(2), 141-52.
Takaoka, Eiji, et al. "Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line." American journal of cancer research vol. 2,2 (2012): 141-52.
Takaoka E, Sonobe H, Akimaru K, Sakamoto S, Shuin T, Daibata M, Taguchi T, Tominaga A. Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line. Am J Cancer Res. 2012;2(2):141-52. Epub 2012 Feb 19. PMID: 22432055; PMCID: PMC3304565.
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Am J Cancer Res. 2012;2(2):141-52. Epub 2012 Feb 19.

Multiple sites of highly amplified DNA sequences detected by molecular cytogenetic analysis in HS-RMS-2, a new pleomorphic rhabdomyosarcoma cell line.

American journal of cancer research

Eiji Takaoka, Hiroshi Sonobe, Kunihiro Akimaru, Shuji Sakamoto, Taro Shuin, Masanori Daibata, Takahiro Taguchi, Akira Tominaga

Affiliations

  1. Division of Human Health and Medical Science, Graduate School of Kuroshio Science, Kochi University, Nankoku, Kochi 783-8505, Japan.

PMID: 22432055 PMCID: PMC3304565

Abstract

A molecular cytogenetic analysis was performed on HS-RMS-2, a cell line established in this laboratory from a rare pleomorphic type of rhabdomyosarcoma. G-banding and multicolor-FISH analyses revealed that the cells have a complex chromosomal composition. Comparative genomic in situ hybridization (CGH) detected eight highly amplified regions at 1p36.1-p36.2, 1p31-p32, 1q21-q31, 8q12-q21, 8q24-qter, 11q12-q13, 12q13-q14 and 18q12-q22, suggesting the co-existence of multiple amplified oncogenes in these tumor cells. Reverse chromosome painting, using a probe regenerated by microdissection of a long marker chromosome, revealed the native location of three of eight possible genes to be on chromosomes 1p31-32, 12q14 and 18q21. FISH using BAC and cosmid probes revealed amplification of JUN (1p31), MYC (8q24), CCND1 (11q13), INT2 (11q13.3), MDM2 (12q14.3-q15) and MALT (18q21). These findings indicate that at least eight amplified oncogenes may contribute to the pathogenesis of a rare pleomorphic type of rhabdomyosarcoma. This new cell line should prove useful for in vitro preclinical studies of molecularly targeted therapies.

Keywords: CGH; FISH; Rhabdomyosarcoma; co-amplification; homogeneously staining region; oncogenes

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