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de Faria O, Cui QL, Bin JM, et al. Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes. Front Genet. 2012;3:46doi: 10.3389/fgene.2012.00046.
de Faria, O., Cui, Q. L., Bin, J. M., Bull, S. J., Kennedy, T. E., Bar-Or, A., Antel, J. P., Colman, D. R., & Dhaunchak, A. S. (2012). Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes. Frontiers in genetics, 346. https://doi.org/10.3389/fgene.2012.00046
de Faria, Omar, et al. "Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes." Frontiers in genetics vol. 3 (2012): 46. doi: https://doi.org/10.3389/fgene.2012.00046
de Faria O, Cui QL, Bin JM, Bull SJ, Kennedy TE, Bar-Or A, Antel JP, Colman DR, Dhaunchak AS. Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes. Front Genet. 2012 Mar 28;3:46. doi: 10.3389/fgene.2012.00046. eCollection 2012. PMID: 22470405; PMCID: PMC3314186.
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Front Genet. 2012 Mar 28;3:46. doi: 10.3389/fgene.2012.00046. eCollection 2012.

Regulation of miRNA 219 and miRNA Clusters 338 and 17-92 in Oligodendrocytes.

Frontiers in genetics

Omar de Faria, Qiao-Ling Cui, Jenea M Bin, Sarah-Jane Bull, Timothy E Kennedy, Amit Bar-Or, Jack P Antel, David R Colman, Ajit S Dhaunchak

Affiliations

  1. Department of Neurology and Neurosurgery, Montreal Neurological Institute and Hospital, McGill University Health Centre, McGill University Montreal, QC, Canada.

PMID: 22470405 PMCID: PMC3314186 DOI: 10.3389/fgene.2012.00046

Abstract

MicroRNAs (miRs) regulate diverse molecular and cellular processes including oligodendrocyte (OL) precursor cell (OPC) proliferation and differentiation in rodents. However, the role of miRs in human OPCs is poorly understood. To identify miRs that may regulate these processes in humans, we isolated OL lineage cells from human white matter and analyzed their miR profile. Using endpoint RT-PCR assays and quantitative real-time PCR, we demonstrate that miR-219, miR-338, and miR-17-92 are enriched in human white matter and expressed in acutely isolated human OLs. In addition, we report the expression of closely related miRs (miR-219-1-3p, miR-219-2-3p, miR-1250, miR-657, miR-3065-5p, miR-3065-3p) in both rodent and human OLs. Our findings demonstrate that miRs implicated in rodent OPC proliferation and differentiation are regulated in human OLs and may regulate myelination program in humans. Thus, these miRs should be recognized as potential therapeutic targets in demyelinating disorders.

Keywords: differentiation; microRNA; myelination; oligodendrocyte precursor cell

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