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Groeneveld E, Tinh NH, Kues W, et al. A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study. Animal. 2008;2(1):1-8doi: 10.1017/S1751731107000869.
Groeneveld, E., Tinh, N. H., Kues, W., & Vien, N. T. (2008). A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study. Animal : an international journal of animal bioscience, 2(1), 1-8. https://doi.org/10.1017/S1751731107000869
Groeneveld, E, et al. "A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study." Animal : an international journal of animal bioscience vol. 2,1 (2008): 1-8. doi: https://doi.org/10.1017/S1751731107000869
Groeneveld E, Tinh NH, Kues W, Vien NT. A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study. Animal. 2008 Jan;2(1):1-8. doi: 10.1017/S1751731107000869. PMID: 22444957.
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Animal. 2008 Jan;2(1):1-8. doi: 10.1017/S1751731107000869.

A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study.

Animal : an international journal of animal bioscience

E Groeneveld, Nguyen Huu Tinh, W Kues, Nguyen Thi Vien

Affiliations

  1. 1Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany.

PMID: 22444957 DOI: 10.1017/S1751731107000869

Abstract

A protocol was developed for collection and storage of somatic cell samples under adverse conditions with little infrastructure, for somatic-cell banks as a backup for endangered livestock breeds. The procedure, which is uniform across species, includes sample collection with ear taggers with an integrated tag/vial system, and recording of global positioning system (GPS) coordinates, digital images and breed information. In a pilot study, the procedures were tested on six local Vietnamese populations of pigs, sheep and goats. Initial investment was around €3000, while the total variable cost for sampling one breed with 25 females and 25 males was less than €1000. With support from local organisations, the sampling of six breeds with 300 animals was carried out in 2 months. The protocol and the complete workflow for setting up a somatic-cell bank, together with data collection, are described. The procedure has proved practicable and exceedingly cheap relative to the cryopreservation of semen or embryos.

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