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Puppa MJ, White JP, Velázquez KT, et al. The effect of exercise on IL-6-induced cachexia in the Apc ( Min/+) mouse. J Cachexia Sarcopenia Muscle. 2011;3(2):117-37doi: 10.1007/s13539-011-0047-1.
Puppa, M. J., White, J. P., Velázquez, K. T., Baltgalvis, K. A., Sato, S., Baynes, J. W., & Carson, J. A. (2012). The effect of exercise on IL-6-induced cachexia in the Apc ( Min/+) mouse. Journal of cachexia, sarcopenia and muscle, 3(2), 117-37. https://doi.org/10.1007/s13539-011-0047-1
Puppa, Melissa J, et al. "The effect of exercise on IL-6-induced cachexia in the Apc ( Min/+) mouse." Journal of cachexia, sarcopenia and muscle vol. 3,2 (2012): 117-37. doi: https://doi.org/10.1007/s13539-011-0047-1
Puppa MJ, White JP, Velázquez KT, Baltgalvis KA, Sato S, Baynes JW, Carson JA. The effect of exercise on IL-6-induced cachexia in the Apc ( Min/+) mouse. J Cachexia Sarcopenia Muscle. 2012 Jun;3(2):117-37. doi: 10.1007/s13539-011-0047-1. Epub 2011 Nov 30. PMID: 22476915; PMCID: PMC3374019.
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J Cachexia Sarcopenia Muscle. 2012 Jun;3(2):117-37. doi: 10.1007/s13539-011-0047-1. Epub 2011 Nov 30.

The effect of exercise on IL-6-induced cachexia in the Apc ( Min/+) mouse.

Journal of cachexia, sarcopenia and muscle

Melissa J Puppa, James P White, Kandy T Velázquez, Kristen A Baltgalvis, Shuichi Sato, John W Baynes, James A Carson

Affiliations

  1. Integrative Muscle Biology Laboratory, Exercise Science Department, University of South Carolina, Columbia, SC, USA.

PMID: 22476915 PMCID: PMC3374019 DOI: 10.1007/s13539-011-0047-1

Abstract

BACKGROUND: Cachexia involves unintentional body weight loss including diminished muscle and adipose tissue mass and is associated with an underlying disease. Systemic overexpression of IL-6 accelerates cachexia in the Apc(Min/+) mouse, but does not induce wasting in control C57BL/6 mice. With many chronic diseases, chronic inflammation and metabolic dysfunction can be improved with moderate exercise. A direct effect of regular moderate exercise on the prevention of IL-6-induced cachexia in the Apc(Min/+) mouse has not been investigated. The purpose of this study was to assess the effects of exercise on the development of cachexia in the Apc(Min/+) mouse.

METHODS: Mice were randomly assigned to moderate treadmill exercise (18 m/min, 1 h, 6 days/week, 5% grade) or cage control (CC) groups from 6 to 14 weeks of age. At 12 weeks of age, mice were electroporated with either IL-6-containing or control plasmid into the quadriceps muscle. Mice were killed after 2 weeks of systemic IL-6 overexpression or control treatment.

RESULTS: IL-6 overexpression induced an 8% loss in body weight in CC mice, which was significantly attenuated by exercise. IL-6 overexpression in CC mice increased fasting insulin and triglyceride levels, which were normalized by exercise, and associated with increased oxidative capacity, an induction of AKT signaling, and a repression of AMPK signaling in muscle. These exercise-induced changes occurred despite elevated inflammatory signaling in skeletal muscle.

CONCLUSION: We conclude that moderate-intensity exercise can attenuate IL-6-dependent cachexia in Apc(Min/+) mice, independent of changes in IL-6 concentration and muscle inflammatory signaling. The exercise effect was associated with improved insulin sensitivity and improved energy status in the muscle.

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