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Iacconi P, Giusti L, Da Valle Y, et al. Proteomic approach used in the diagnosis of Riedel's thyroiditis: a case report. J Med Case Rep. 2012;6:103doi: 10.1186/1752-1947-6-103.
Iacconi, P., Giusti, L., Da Valle, Y., Ciregia, F., Giannaccini, G., Torregrossa, L., Proietti, A., Donatini, G., Mazzeo, S., Basolo, F., & Lucacchini, A. (2012). Proteomic approach used in the diagnosis of Riedel's thyroiditis: a case report. Journal of medical case reports, 6103. https://doi.org/10.1186/1752-1947-6-103
Iacconi, Pietro, et al. "Proteomic approach used in the diagnosis of Riedel's thyroiditis: a case report." Journal of medical case reports vol. 6 (2012): 103. doi: https://doi.org/10.1186/1752-1947-6-103
Iacconi P, Giusti L, Da Valle Y, Ciregia F, Giannaccini G, Torregrossa L, Proietti A, Donatini G, Mazzeo S, Basolo F, Lucacchini A. Proteomic approach used in the diagnosis of Riedel's thyroiditis: a case report. J Med Case Rep. 2012 Apr 05;6:103. doi: 10.1186/1752-1947-6-103. PMID: 22480342; PMCID: PMC3349472.
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J Med Case Rep. 2012 Apr 05;6:103. doi: 10.1186/1752-1947-6-103.

Proteomic approach used in the diagnosis of Riedel's thyroiditis: a case report.

Journal of medical case reports

Pietro Iacconi, Laura Giusti, Ylenia Da Valle, Federica Ciregia, Gino Giannaccini, Liborio Torregrossa, Agnese Proietti, Gianluca Donatini, Salvatore Mazzeo, Fulvio Basolo, Antonio Lucacchini

Affiliations

  1. Department of Psychiatry, Neurobiology, Pharmacology and Biotechnology, Via Bonanno 6, University of Pisa, 56126 Pisa, Italy. [email protected].

PMID: 22480342 PMCID: PMC3349472 DOI: 10.1186/1752-1947-6-103

Abstract

INTRODUCTION: Riedel's thyroiditis, a rare thyroid disease, can be difficult to diagnose prior to surgical removal and can be confused with malignancy both clinically and cytologically.

CASE PRESENTATION: We report the case of a 72-year-old Caucasian woman who presented with a goiter, which showed a rapid increase in size at ultrasound check, suggesting malignancy. Because of inconclusive cytology, a total thyroidectomy was performed. Fine-needle aspiration of the removed thyroid was processed by two-dimensional electrophoresis, and the proteome was compared with both anaplastic cancer and control samples. Significant differentially expressed protein spots were identified by Western blot analysis by using specific antibodies.

CONCLUSIONS: The protein pattern of Riedel's fine-needle aspiration revealed a superimposition with that of the control samples. The comparison of the protein pattern of Riedel's thyroiditis fine-needle aspiration with that of anaplastic cancer showed evidence of a different expression of ferritin heavy chains, ferritin light chains, and haptoglobins, as previously reported in thyroid cancers. Therefore, we performed Western blot analysis of these proteins and validated that their expression levels were low or absent in Riedel's thyroiditis and control samples despite the high concentrations present in fine-needle aspiration anaplastic samples. The concurrent absent or low expression levels of haptoglobin, ferritin light chain, and ferritin heavy chain in Riedel's thyroiditis fine-needle aspiration samples strongly indicate the benign nature of the thyroid lesion. These results suggest the potential applicability of fine-needle aspiration proteome analysis for Riedel's thyroiditis diagnosis.

References

  1. Electrophoresis. 2000 Nov;21(17):3666-72 - PubMed
  2. Thyroid. 2005 Jan;15(1):85-8 - PubMed
  3. Anal Chem. 2002 Oct 15;74(20):5383-92 - PubMed
  4. Am J Clin Pathol. 1988 Dec;90(6):715-22 - PubMed
  5. J Med Case Rep. 2010 Jan 20;4:15 - PubMed
  6. Pathol Annu. 1991;26 Pt 1:63-91 - PubMed
  7. Anal Chem. 2003 Sep 1;75(17):4646-58 - PubMed
  8. Int J Clin Pract. 2004 Mar;58(3):312-5 - PubMed
  9. Int J Clin Pract. 2002 Jan-Feb;56(1):65-7 - PubMed
  10. Mol Biosyst. 2011 Mar;7(3):687-99 - PubMed
  11. J Endocrinol Invest. 2007 Nov;30(10):865-9 - PubMed
  12. Croat Med J. 2003 Apr;44(2):239-41 - PubMed
  13. Arch Otolaryngol Head Neck Surg. 1991 Feb;117(2):214-7 - PubMed
  14. Ann Intern Med. 1967 May;66(5):884-92 - PubMed
  15. Mayo Clin Proc. 1985 Dec;60(12):836-43 - PubMed
  16. J Proteome Res. 2008 Sep;7(9):4079-88 - PubMed

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