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Marmotti A, Mattia S, Bruzzone M, et al. Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study. Stem Cells Int. 2012;2012:326813doi: 10.1155/2012/326813.
Marmotti, A., Mattia, S., Bruzzone, M., Buttiglieri, S., Risso, A., Bonasia, D. E., Blonna, D., Castoldi, F., Rossi, R., Zanini, C., Ercole, E., Defabiani, E., Tarella, C., & Peretti, G. M. (2012). Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study. Stem cells international, 2012326813. https://doi.org/10.1155/2012/326813
Marmotti, A, et al. "Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study." Stem cells international vol. 2012 (2012): 326813. doi: https://doi.org/10.1155/2012/326813
Marmotti A, Mattia S, Bruzzone M, Buttiglieri S, Risso A, Bonasia DE, Blonna D, Castoldi F, Rossi R, Zanini C, Ercole E, Defabiani E, Tarella C, Peretti GM. Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study. Stem Cells Int. 2012;2012:326813. doi: 10.1155/2012/326813. Epub 2012 Mar 07. PMID: 22550503; PMCID: PMC3328184.
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Stem Cells Int. 2012;2012:326813. doi: 10.1155/2012/326813. Epub 2012 Mar 07.

Minced umbilical cord fragments as a source of cells for orthopaedic tissue engineering: an in vitro study.

Stem cells international

A Marmotti, S Mattia, M Bruzzone, S Buttiglieri, A Risso, D E Bonasia, D Blonna, F Castoldi, R Rossi, C Zanini, E Ercole, E Defabiani, C Tarella, G M Peretti

Affiliations

  1. Department of Orthopaedics and Traumatology, University of Turin, Mauriziano Hospital, Largo Turati 62, 10128 Turin, Italy.

PMID: 22550503 PMCID: PMC3328184 DOI: 10.1155/2012/326813

Abstract

A promising approach for musculoskeletal repair and regeneration is mesenchymal-stem-cell- (MSC-)based tissue engineering. The aim of the study was to apply a simple protocol based on mincing the umbilical cord (UC), without removing any blood vessels or using any enzymatic digestion, to rapidly obtain an adequate number of multipotent UC-MSCs. We obtained, at passage 1 (P1), a mean value of 4, 2 × 10(6) cells (SD 0,4) from each UC. At immunophenotypic characterization, cells were positive for CD73, CD90, CD105, CD44, CD29, and HLA-I and negative for CD34 and HLA-class II, with a subpopulation negative for both HLA-I and HLA-II. Newborn origin and multilineage potential toward bone, fat, cartilage, and muscle was demonstrated. Telomere length was similar to that of bone-marrow (BM) MSCs from young donors. The results suggest that simply collecting UC-MSCs at P1 from minced umbilical cord fragments allows to achieve a valuable population of cells suitable for orthopaedic tissue engineering.

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