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Holmes RS, Vandeberg JL, Cox LA. Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H). 3 Biotech. 2011;1(2):99-109doi: 10.1007/s13205-011-0013-9.
Holmes, R. S., Vandeberg, J. L., & Cox, L. A. (2011). Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H). 3 Biotech, 1(2), 99-109. https://doi.org/10.1007/s13205-011-0013-9
Holmes, Roger S, et al. "Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H)." 3 Biotech vol. 1,2 (2011): 99-109. doi: https://doi.org/10.1007/s13205-011-0013-9
Holmes RS, Vandeberg JL, Cox LA. Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H). 3 Biotech. 2011 Sep;1(2):99-109. doi: 10.1007/s13205-011-0013-9. Epub 2011 Aug 03. PMID: 22582164; PMCID: PMC3324826.
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3 Biotech. 2011 Sep;1(2):99-109. doi: 10.1007/s13205-011-0013-9. Epub 2011 Aug 03.

Genomics and proteomics of vertebrate cholesterol ester lipase (LIPA) and cholesterol 25-hydroxylase (CH25H).

3 Biotech

Roger S Holmes, John L Vandeberg, Laura A Cox

PMID: 22582164 PMCID: PMC3324826 DOI: 10.1007/s13205-011-0013-9

Abstract

Cholesterol ester lipase (LIPA; EC 3.1.1.13) and cholesterol 25-hydroxylase (CH25H; EC 1.14.99.48) play essential role in cholesterol metabolism in the body by hydrolysing cholesteryl esters and triglycerides within lysosomes (LIPA) and catalysing the formation of 25-hydroxycholesterol from cholesterol (CH25H) which acts to repress cholesterol biosynthesis. Bioinformatic methods were used to predict the amino acid sequences, structures and genomic features of several vertebrate LIPA and CH25H genes and proteins, and to examine the phylogeny of vertebrate LIPA. Amino acid sequence alignments and predicted subunit structures enabled the identification of key sequences previously reported for human LIPA and CH25H and transmembrane structures for vertebrate CH25H sequences. Vertebrate LIPA and CH25H genes were located in tandem on all vertebrate genomes examined and showed several predicted transcription factor binding sites and CpG islands located within the 5' regions of the human genes. Vertebrate LIPA genes contained nine coding exons, while all vertebrate CH25H genes were without introns. Phylogenetic analysis demonstrated the distinct nature of the vertebrate LIPA gene and protein family in comparison with other vertebrate acid lipases and has apparently evolved from an ancestral LIPA gene which predated the appearance of vertebrates. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-011-0013-9) contains supplementary material, which is available to authorized users.

References

  1. J Biol Chem. 2002 Jan 18;277(3):2266-74 - PubMed
  2. Am J Hum Genet. 1992 Jun;50(6):1275-80 - PubMed
  3. Nat Genet. 1999 Apr;21(4):424-8 - PubMed
  4. Mol Biol Evol. 1987 Jul;4(4):406-25 - PubMed
  5. J Biol Chem. 1991 Nov 25;266(33):22479-84 - PubMed
  6. J Biol Chem. 2007 Mar 23;282(12):8959-68 - PubMed
  7. Genome Res. 2002 Jun;12(6):996-1006 - PubMed
  8. Proc Natl Acad Sci U S A. 1994 Mar 29;91(7):2718-22 - PubMed
  9. Genome Biol. 2006;7 Suppl 1:S12.1-14 - PubMed
  10. J Biol Chem. 1999 Jun 11;274(24):16995-7002 - PubMed
  11. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed
  12. J Biol Chem. 1975 Nov 10;250(21):8487-95 - PubMed
  13. Nature. 2004 May 27;429(6990):375-81 - PubMed
  14. Biochim Biophys Acta. 1987 Aug 25;909(3):237-44 - PubMed
  15. Nat Protoc. 2007;2(4):953-71 - PubMed
  16. Mol Cell Proteomics. 2006 Apr;5(4):686-701 - PubMed
  17. Am J Hum Genet. 1984 Nov;36(6):1190-203 - PubMed
  18. J Biochem. 2004 Jul;136(1):65-72 - PubMed
  19. Neurobiol Aging. 2004 Nov-Dec;25(10):1305-8 - PubMed
  20. Neurosci Lett. 2006 Jan 2;391(3):142-6 - PubMed
  21. J Biol Chem. 1999 May 14;274(20):14170-5 - PubMed
  22. Nature. 2004 Dec 9;432(7018):695-716 - PubMed
  23. Biochemistry. 1994 Nov 1;33(43):12776-86 - PubMed
  24. J Mol Biol. 1998 Mar 13;276(5):877-85 - PubMed
  25. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16764-9 - PubMed
  26. Nature. 2005 Sep 1;437(7055):69-87 - PubMed
  27. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1412-7 - PubMed
  28. Trends Ecol Evol. 2007 Aug;22(8):424-31 - PubMed
  29. Comput Appl Biosci. 1994 Sep;10(5):569-70 - PubMed
  30. Science. 2007 Apr 13;316(5822):222-34 - PubMed
  31. Eur J Biochem. 1994 Feb 1;219(3):905-14 - PubMed
  32. Somatic Cell Genet. 1981 May;7(3):345-58 - PubMed
  33. Science. 1987 Mar 27;235(4796):1638-41 - PubMed
  34. Biochemistry. 1989 Nov 14;28(23):8966-71 - PubMed
  35. J Mol Biol. 2001 Jan 19;305(3):567-80 - PubMed
  36. Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1455-61 - PubMed
  37. J Biol Chem. 1988 Aug 5;263(22):10907-14 - PubMed
  38. J Biol Chem. 1998 Dec 18;273(51):34316-27 - PubMed
  39. Comp Biochem Physiol Part D Genomics Proteomics. 2010 Sep;5(3):217-26 - PubMed
  40. Nucleic Acids Res. 2004 Jan 1;32(Database issue):D230-4 - PubMed
  41. Nucleic Acids Res. 2007 Jan;35(Database issue):D610-7 - PubMed
  42. Evolution. 1985 Jul;39(4):783-791 - PubMed
  43. Nature. 2004 Apr 1;428(6982):493-521 - PubMed
  44. Am J Hum Genet. 1981 Mar;33(2):203-8 - PubMed
  45. Physiol Genomics. 2005 Nov 17;23(3):304-10 - PubMed
  46. J Lipid Res. 1996 May;37(5):937-49 - PubMed
  47. Genome Biol. 2007;8(6):R107 - PubMed
  48. Nature. 2002 Dec 5;420(6915):520-62 - PubMed
  49. J Pediatr. 1977 Jun;90(6):910-4 - PubMed

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