Display options
Cite
Roslan HA, Anji SB. Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display. 3 Biotech. 2011;1(1):27-33doi: 10.1007/s13205-011-0004-x.
Roslan, H. A., & Anji, S. B. (2011). Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display. 3 Biotech, 1(1), 27-33. https://doi.org/10.1007/s13205-011-0004-x
Roslan, Hairul Azman, and Anji, Syahrul Bariyah. "Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display." 3 Biotech vol. 1,1 (2011): 27-33. doi: https://doi.org/10.1007/s13205-011-0004-x
Roslan HA, Anji SB. Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display. 3 Biotech. 2011 Jul;1(1):27-33. doi: 10.1007/s13205-011-0004-x. Epub 2011 May 03. PMID: 22558533; PMCID: PMC3339612.
Copy Download .nbib Format: NLM
Share it on

3 Biotech. 2011 Jul;1(1):27-33. doi: 10.1007/s13205-011-0004-x. Epub 2011 May 03.

Characterization of inflorescence-predominant chitinase gene in Metroxylon sagu via differential display.

3 Biotech

Hairul Azman Roslan, Syahrul Bariyah Anji

Affiliations

  1. Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak Malaysia.

PMID: 22558533 PMCID: PMC3339612 DOI: 10.1007/s13205-011-0004-x

Abstract

Chitinase is an enzyme that catalyzes the degradation of chitin, commonly induced upon the attack of pathogens and other stresses. A cDNA (MsChi1) was isolated from Metroxylon sagu and expressed predominantly in the inflorescence tissue of M. sagu, suggesting its role in developmental processes. The chitinase cDNA was detected and isolated via differential display and rapid amplification of cDNA ends (RACE). Primers specific to M. saguchitinase were used as probes to amplify the 3'-end and 5'-end regions of chitinase cDNA. Transcript analysis showed that chitinase is expressed in inflorescence and meristem tissues but was not detected in the leaf tissue. Sequence analysis of amplified cDNA fragments of 3'-end and 5'-end regions indicated that the chitinase cDNA was successfully amplified. The M. saguchitinase cDNA isolated was approximately 1,143 bp long and corresponds to 312 predicted amino acids. Alignments of nucleotide and amino acid have grouped this chitinase to family 19 class I chitinase.

References

  1. Planta. 1983 Feb;157(1):22-31 - PubMed
  2. Biol Chem. 2000 Aug;381(8):667-78 - PubMed
  3. Proc Natl Acad Sci U S A. 2000 May 9;97(10):5322-7 - PubMed
  4. Plant Mol Biol. 1996 Mar;30(5):899-911 - PubMed
  5. Plant Mol Biol. 1990 Feb;14(2):119-26 - PubMed
  6. J Biosci Bioeng. 2002;94(6):526-9 - PubMed
  7. Biotechniques. 2004 Mar;36(3):424-6, 428, 430 passim - PubMed
  8. Plant Cell. 1990 Jul;2(7):673-84 - PubMed
  9. Proc Natl Acad Sci U S A. 1988 Dec;85(23):8998-9002 - PubMed
  10. Plant Cell. 1989 Sep;1(9):881-7 - PubMed
  11. Planta. 1994;194(2):264-73 - PubMed
  12. Plant Physiol. 1998 May;117(1):43-53 - PubMed
  13. Plant Mol Biol. 1998 Feb;36(3):407-15 - PubMed
  14. Planta. 1999 Aug 12;209(2):195-201 - PubMed
  15. Biochim Biophys Acta. 1995 Sep 19;1263(3):212-20 - PubMed
  16. Planta. 1997;201(2):189-94 - PubMed
  17. Plant Cell. 1991 Oct;3(10):1063-1072 - PubMed
  18. Plant J. 1994 Oct;6(4):579-89 - PubMed
  19. Plant Physiol. 1990 Jul;93(3):907-14 - PubMed
  20. Planta. 2001 Mar;212(4):556-67 - PubMed
  21. Nucleic Acids Res. 2011 Jan;39(Database issue):D225-9 - PubMed
  22. Anal Biochem. 1989 May 1;178(2):362-6 - PubMed
  23. Biosci Biotechnol Biochem. 2005 Apr;69(4):852-4 - PubMed
  24. Plant Mol Biol. 2000 Apr;42(6):883-97 - PubMed
  25. Plant J. 1993 Jan;3(1):31-40 - PubMed
  26. J Exp Bot. 2001 Jan;52(354):1-9 - PubMed

Publication Types