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Riess D, Lavrik I. 2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC. Acta Naturae. 2010;2(2):96-102
Riess, D., & Lavrik, I. (2010). 2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC. Acta naturae, 2(2), 96-102.
Riess, D, and Lavrik, I. "2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC." Acta naturae vol. 2,2 (2010): 96-102.
Riess D, Lavrik I. 2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC. Acta Naturae. 2010 Jul;2(2):96-102. PMID: 22649647; PMCID: PMC3347551.
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Acta Naturae. 2010 Jul;2(2):96-102.

2D-gel Electrophoresis As a Tool to Investigate the Composition of CD95 DISC.

Acta naturae

D Riess, I Lavrik

Affiliations

  1. Division of Immunogenetics, German Cancer Center, Heidelberg, Germany.

PMID: 22649647 PMCID: PMC3347551

Abstract

Stimulation of CD95 (APO-1/Fas) leads to apoptosis induction in multicellular organisms. CD95-mediated apoptosis starts with the formation of the protein complex at the receptor CD95 (APO-1/Fas), which was named DISC (death-inducing signaling complex). In this work, the composition of the CD95 DISC in two different cell types was analyzed using proteomics approaches. Using 2D gels, the composition of the CD95 DISC was analyzed in the so-called Type I and Type II cells, which are characterized by different kinetics of apoptosis. The detailed analysis of the CD95 DISC performed by 2D gels demonstrated that, besides the well-established components of the CD95 DISC, which are present in both cell types (CD95, FADD and procaspase-8), there are a number of differential spots detected at the CD95 DISC of Type I versus Type II cells. Taken together, this work demonstrates the differential composition of the CD95 DISC of Type I versus Type II cells.

Keywords: 2D-gel electrophoresis; apoptosis; receptor CD95

References

  1. Cell Death Differ. 2003 Jan;10(1):26-35 - PubMed
  2. Methods Mol Biol. 1999;112:197-209 - PubMed
  3. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
  4. Nat Med. 1999 Feb;5(2):157-63 - PubMed
  5. J Biol Chem. 1997 Oct 24;272(43):26953-8 - PubMed
  6. Cell Death Differ. 2006 Mar;13(3):489-98 - PubMed
  7. J Cell Sci. 2005 Jan 15;118(Pt 2):265-7 - PubMed
  8. Science. 1989 Jul 21;245(4915):301-5 - PubMed
  9. Nature. 2000 Oct 12;407(6805):789-95 - PubMed
  10. EMBO J. 1995 Nov 15;14(22):5579-88 - PubMed
  11. Electrophoresis. 1988 Sep;9(9):531-46 - PubMed
  12. J Biol Chem. 2005 Apr 15;280(15):14507-13 - PubMed

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