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Abarikwu SO, Otuechere CA, Ekor M, et al. Rutin Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats. Toxicol Int. 2012;19(2):207-14doi: 10.4103/0971-6580.97224.
Abarikwu, S. O., Otuechere, C. A., Ekor, M., Monwuba, K., & Osobu, D. (2012). Rutin Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats. Toxicology international, 19(2), 207-14. https://doi.org/10.4103/0971-6580.97224
Abarikwu, S O, et al. "Rutin Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats." Toxicology international vol. 19,2 (2012): 207-14. doi: https://doi.org/10.4103/0971-6580.97224
Abarikwu SO, Otuechere CA, Ekor M, Monwuba K, Osobu D. Rutin Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats. Toxicol Int. 2012 May;19(2):207-14. doi: 10.4103/0971-6580.97224. PMID: 22778522; PMCID: PMC3388768.
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Toxicol Int. 2012 May;19(2):207-14. doi: 10.4103/0971-6580.97224.

Rutin Ameliorates Cyclophosphamide-induced Reproductive Toxicity in Male Rats.

Toxicology international

S O Abarikwu, C A Otuechere, M Ekor, K Monwuba, D Osobu

Affiliations

  1. Department of Chemical Sciences, College of Natural Sciences, Redeemer's University, Redemption City, Mowe, Ogun State, Nigeria.

PMID: 22778522 PMCID: PMC3388768 DOI: 10.4103/0971-6580.97224

Abstract

Cyclophosphamide (CYC) as an anticancer alkylating agent has been known as a male reproductive toxicant. This study was aimed to evaluate the protective effect of rutin (RUT) on CYC-induced reproductive toxicity. Sexually mature Wistar rats (weighing 199 ± 10 g with five animals in each group) were given CYC (15 mg/kg) and/or RUT (30 mg/kg) twice a week via gavage for 4 weeks. The sperm counts, sperm motility, sperm morphology, daily sperm production (DSP), testicular, and epididymal antioxidant systems: superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), glutathione reductase (GR), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), and testicular steroidogenic enzymes (3β-hydroxysteroid dehydrogenase and 17β-HSD and spermatogenesis marker enzymes (lactate dehydrogenase (LDH), sorbitol dehydrogenase (SDH), alkaline phosphatase (ALP), acid phosphatase (ACP) in the testes, epididymis and seminal vesicles were investigated at the end of the fourth week. By the end of the fourth week, RUT prevented lower sperm counts, sperm motility, DSP, and higher abnormal sperm numbers induced by CYC. In testes, RUT decreased SOD, LDH, and SDH and increased CAT, 3β-HSD, 17β-HSD, ALP, and ACP induced by CYC. In epididymis, RUT increased SOD, CAT, GSH, GSH-Px, GR, GST SDH, ALP and ACP and decreased MDA and LDH induced by CYC. In seminal vesicles, marker enzymes were unchanged in rats given CYC alone or in combination with RUT. It appears that RUT ameliorates CYC reproductive toxicity at the investigated dose.

Keywords: Antioxidant system; cyclophosphamide; rats; reproductive toxicity; rutin; sperms

References

  1. Hum Exp Toxicol. 2008 Dec;27(12):901-10 - PubMed
  2. Reprod Fertil Dev. 2010;22(4):587-96 - PubMed
  3. APMIS. 1998 Jan;106(1):37-45; discussion 45-6 - PubMed
  4. J Appl Toxicol. 2003 Jul-Aug;23(4):271-4 - PubMed
  5. Arch Environ Contam Toxicol. 2010 Apr;58(3):874-82 - PubMed
  6. Toxicology. 2010 Apr 30;271(1-2):51-6 - PubMed
  7. J Pineal Res. 2006 Aug;41(1):21-7 - PubMed
  8. Reprod Toxicol. 2004 Dec;19(2):163-7 - PubMed
  9. Am J Kidney Dis. 2004 May;43(5):772-81 - PubMed
  10. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  11. Anal Biochem. 1972 Jun;47(2):389-94 - PubMed
  12. Toxicol In Vitro. 2011 Jun;25(4):848-58 - PubMed
  13. Arch Biochem Biophys. 1998 Jul 1;355(1):43-8 - PubMed
  14. Biosci Biotechnol Biochem. 2008 May;72(5):1235-41 - PubMed
  15. Biochim Biophys Acta. 1969 Jul 8;185(1):39-48 - PubMed
  16. Toxicol Sci. 2005 Dec;88(2):545-50 - PubMed
  17. Hum Reprod Update. 2001 Jul-Aug;7(4):363-9 - PubMed
  18. Endocrinology. 2003 Jul;144(7):2882-91 - PubMed
  19. Braz J Med Biol Res. 2000 Nov;33(11):1335-42 - PubMed
  20. Toxicology. 2002 Aug 15;177(2-3):187-96 - PubMed
  21. J Biol Chem. 1972 May 25;247(10):3170-5 - PubMed
  22. Curr Top Med Chem. 2001 Dec;1(6):569-90 - PubMed
  23. Mech Ageing Dev. 1990 Feb 15;51(3):283-97 - PubMed
  24. Curr Opin Genet Dev. 2003 Feb;13(1):90-6 - PubMed
  25. Anal Biochem. 1979 Jun;95(2):351-8 - PubMed
  26. Asian J Androl. 2002 Sep;4(3):201-7 - PubMed
  27. Exp Toxicol Pathol. 2011 Mar;63(3):201-7 - PubMed
  28. J Biochem Mol Toxicol. 2008 Sep-Oct;22(5):345-53 - PubMed
  29. Mol Hum Reprod. 2002 Feb;8(2):109-17 - PubMed
  30. Clin Chim Acta. 2006 May;367(1-2):114-9 - PubMed
  31. Science. 1973 Feb 9;179(4073):588-90 - PubMed
  32. Int J Androl. 1997 Apr;20(2):86-91 - PubMed
  33. Am J Clin Pathol. 1958 Sep;30(3):234-6 - PubMed
  34. Pharmacology. 1974;11(3):151-69 - PubMed
  35. Curr Med Chem. 2001 Jun;8(7):797-807 - PubMed
  36. Pharmacol Rep. 2005 Jan-Feb;57(1):84-9 - PubMed
  37. J Natl Cancer Inst. 1999 Feb 3;91(3):215-25 - PubMed
  38. J Biol Chem. 1974 Nov 25;249(22):7130-9 - PubMed

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