J Clin Med Res. 2012 Jun;4(3):190-6. doi: 10.4021/jocmr909w. Epub 2012 May 15.
Ultrastructural Changes in Rat's Atrial Cardiomyocytes After Short Term Administration of Amiodarone and Possible Protective Role of Vitamin E.
Journal of clinical medicine research
Abdullah S Shatoor, Mohamed Samir Ahmad Zaki, Refaat A Eid, Mohamed A Sayed-Ahmad
Affiliations
Affiliations
- Internal Medicine Department, Cardiology Section, Faculty of Medicine, King Khalid University, Saudi Arabia.
PMID: 22719805
PMCID: PMC3376877 DOI: 10.4021/jocmr909w
Abstract
BACKGROUND: Amiodarone chlorhydrate is a diiodated benzofuran derivative used to treat a variety of cardiac rhythm abnormalities. The use of amiodarone is associated with ultrastructural changes affecting body tissues, but its effect on the ultrastructure of the heart has not yet been fully elucidated.
METHODS: The aim of this study is to test the adverse effects of amiodarone administration on cardiomyocytes and to study the possible protective role of vitamin E co-administration. A total of 18 adult male albino rats were used in this study. The rats were divided randomly into three groups of 6 rats each as follows: group I was considered the control group and was given vegetable oil; group II received 54 mg/kg of oral amiodarone; and group III received a single dose of combined vitamin E (50 mg/kg) and amiodarone (54 mg/kg). After 2 weeks, the rats were sacrificed, and the atrial tissues were harvested and processed for electron microscopic study.
RESULTS: ADMINISTRATION OF AMIODARONE ALONE MODIFIED THE ATRIAL ARCHITECTURE, WHICH WAS DEMONSTRATED BY THE FOLLOWING: mitochondrial enlargement and cristae lysis; marked heterogeneity of myofibril patterns with partial necrosis and disintegration of myofilaments; and irregularities of the sarcomere and less concentration of atrionatriuretic factor (ANF) granules, which localised in closed proximity to the nucleus with disrupted chromatin contents. Concomitant administration of vitamin E with amiodarone showed a considerable preservation of the atrial architecture.
CONCLUSIONS: Oral administration of amiodarone in rats resulted in ultrastructural changes in atria, which can be attenuated by vitamin E co-administration.
Keywords: Amiodarone; Cardiomyocytes; Electron microscopy
References
- Optometry. 2006 Feb;77(2):76-81 - PubMed
- J Pharmacol Exp Ther. 1990 Dec;255(3):1371-6 - PubMed
- Toxicology. 1999 Apr 15;133(2-3):75-84 - PubMed
- J Am Coll Cardiol. 1983 Dec;2(6):1114-28 - PubMed
- Exp Anim. 1996 Apr;45(2):125-33 - PubMed
- N Engl J Med. 1995 Jul 13;333(2):77-82 - PubMed
- Biochim Biophys Acta. 1998 Aug 10;1366(1-2):53-67 - PubMed
- Heart. 2006 Oct;92(10):1395 - PubMed
- J Vet Intern Med. 2000 Jan-Feb;14(1):90-5 - PubMed
- J Pharmacol Exp Ther. 2003 Jan;304(1):277-83 - PubMed
- J Cardiovasc Electrophysiol. 1993 Apr;4(2):161-77 - PubMed
- J Vet Med Sci. 1991 Aug;53(4):561-8 - PubMed
- Cardiovasc Res. 2001 Sep;51(4):736-48 - PubMed
- J Cardiovasc Pharmacol. 1988 Jul;12(1):23-36 - PubMed
- Circulation. 1999 Nov 9;100(19):2025-34 - PubMed
- J Pharmacol Exp Ther. 1990 Dec;255(3):1377-84 - PubMed
- J Am Coll Cardiol. 2001 Nov 15;38(6):1734-40 - PubMed
- Ann Intern Med. 1997 Jan 1;126(1):63-73 - PubMed
- Clin Exp Pharmacol Physiol. 1976 Sep-Oct;3(5):427-42 - PubMed
- Hum Pathol. 1990 Jan;21(1):59-67 - PubMed
- Clin Res Cardiol. 2010 Nov;99(11):693-700 - PubMed
- Jikken Dobutsu. 1992 Jul;41(3):321-8 - PubMed
- Lancet. 1994 Aug 20;344(8921):493-8 - PubMed
- Am J Physiol Heart Circ Physiol. 2004 Jun;286(6):H2169-82 - PubMed
- Saudi J Gastroenterol. 2009 Apr;15(2):104-10 - PubMed
- Circulation. 1991 Feb;83(2):504-14 - PubMed
- J Toxicol Sci. 1996 Nov;21(4):253-67 - PubMed
- Am Heart J. 1983 Oct;106(4 Pt 2):788-97 - PubMed
- Can J Physiol Pharmacol. 1994 Jun;72(6):613-21 - PubMed
- N Engl J Med. 1987 Feb 19;316(8):455-66 - PubMed
- Circulation. 1996 Jun 15;93(12):2128-34 - PubMed
- Circulation. 2001 Mar 6;103(9):1317-24 - PubMed
- Ann Intern Med. 1988 Nov 15;109(10):838-40 - PubMed
- J Nutr. 2002 Oct;132(10):3178-85 - PubMed
- Drug Saf. 2010 Jul 1;33(7):539-58 - PubMed
- J Hepatol. 2001 Nov;35(5):628-36 - PubMed
- J Clin Pharmacol. 1984 Apr;24(4):129-47 - PubMed
- Recent Adv Stud Cardiac Struct Metab. 1976;9:395-419 - PubMed
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