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Shah S, Asnani A, Kawade D, et al. Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique. J Young Pharm. 2012;4(2):88-94doi: 10.4103/0975-1483.96622.
Shah, S., Asnani, A., Kawade, D., Dangre, S., Arora, S., & Yende, S. (2012). Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique. Journal of young pharmacists : JYP, 4(2), 88-94. https://doi.org/10.4103/0975-1483.96622
Shah, S, et al. "Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique." Journal of young pharmacists : JYP vol. 4,2 (2012): 88-94. doi: https://doi.org/10.4103/0975-1483.96622
Shah S, Asnani A, Kawade D, Dangre S, Arora S, Yende S. Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique. J Young Pharm. 2012 Apr;4(2):88-94. doi: 10.4103/0975-1483.96622. PMID: 22754260; PMCID: PMC3385223.
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J Young Pharm. 2012 Apr;4(2):88-94. doi: 10.4103/0975-1483.96622.

Simultaneous Quantitative Analysis of Olmesartan Medoxomil and Amlodipine Besylate in Plasma by High-performance Liquid Chromatography Technique.

Journal of young pharmacists : JYP

S Shah, A Asnani, D Kawade, S Dangre, S Arora, S Yende

Affiliations

  1. Department of Pharmaceutical Chemistry, Smt. Kishoritai Bhoyar College of Pharmacy, Nagpur, India.

PMID: 22754260 PMCID: PMC3385223 DOI: 10.4103/0975-1483.96622

Abstract

A rapid, simple and sensitive high-performance liquid chromatography (HPLC) method has been developed for quantification of olmesartan medoxomil (OLM) and amlodipine besylate (AM) in plasma. The assay enables the measurement of OLM and AM for therapeutic drug monitoring with a minimum detectable limit of 2 ng mL. The method involves a simple, one-step extraction procedure and analytical recovery was above 50%. The separation was performed on an analytical 250 × 4.6 mm Eurospher 100(-5) C18 column. The wavelength was set at 239 nm. The mobile phase was a mixture of acetonitrile:0.05 M ammonium acetate buffer: 0.1 mL triethylamine at pH 6.8 was selected at a flow rate of 1.0 mL min. The calibration curve for the determination of OLM and AM in plasma was linear over the range 2-2500 and 8-10,000 ng mL AM and OLM. The coefficients of variation for interday and intraday assay were found to be <15%. The method can be applied to a pharmacokinetic and pharmacodynamic study of OLM and AM in a combined dosage form.

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