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Biotechnol Res Int. 2012;2012:904763. doi: 10.1155/2012/904763. Epub 2012 Jul 15.

Experimental Investigations on the Effects of Carbon and Nitrogen Sources on Concomitant Amylase and Polygalacturonase Production by Trichoderma viride BITRS-1001 in Submerged Fermentation.

Biotechnology research international

Arotupin Daniel Juwon, Ogunmolu Funso Emmanuel

Affiliations

  1. Department of Microbiology, Federal University of Technology Akure, PMB 704, Akure, Ondo State, Nigeria.

PMID: 22919504 PMCID: PMC3418636 DOI: 10.1155/2012/904763

Abstract

The paper investigates the effects of different commercial carbon and nitrogen sources on the concomitant synthesis of amylase and polygalacturonase enzymes with the aim of optimizing them for maximal enzyme production. The microorganism used in this work was the fungus Trichoderma viride BITRS-1001, which had been previously identified as a highly active producer of amylase and polygalacturonase enzymes. The results showed that the different commercial carbon and nitrogen substrate significantly affected the concomitant syntheses of amylase and polygalacturonase in culture media supplemented with the different commercial carbon and nitrogen substrates. The result obtained suggested that for optimal and concomitant synthesis of the enzymes by Trichoderma viride BITRS-1001 in submerged fermentation, minimal medium supplemented with maltose and casein were the carbon and nitrogen substrates of choice.

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