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Celotto AM, Liu Z, Vandemark AP, et al. A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease. Brain Behav. 2012;2(4):424-34doi: 10.1002/brb3.73.
Celotto, A. M., Liu, Z., Vandemark, A. P., & Palladino, M. J. (2012). A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease. Brain and behavior, 2(4), 424-34. https://doi.org/10.1002/brb3.73
Celotto, Alicia M, et al. "A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease." Brain and behavior vol. 2,4 (2012): 424-34. doi: https://doi.org/10.1002/brb3.73
Celotto AM, Liu Z, Vandemark AP, Palladino MJ. A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease. Brain Behav. 2012 Jul;2(4):424-34. doi: 10.1002/brb3.73. Epub 2012 Jun 25. PMID: 22950046; PMCID: PMC3432965.
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Brain Behav. 2012 Jul;2(4):424-34. doi: 10.1002/brb3.73. Epub 2012 Jun 25.

A novel Drosophila SOD2 mutant demonstrates a role for mitochondrial ROS in neurodevelopment and disease.

Brain and behavior

Alicia M Celotto, Zhaohui Liu, Andrew P Vandemark, Michael J Palladino

PMID: 22950046 PMCID: PMC3432965 DOI: 10.1002/brb3.73

Abstract

Reactive oxygen species (ROS) play essential roles in cell signaling, survival, and homeostasis. Aberrant ROS lead to disease and contribute to the aging process. Numerous enzymes and vigilant antioxidant pathways are required to regulate ROS for normal cellular health. Mitochondria are a major source of ROS, and mechanisms to prevent elevated ROS during oxidative phosphorylation require super oxide dismutase (SOD) activity. SOD2, also known as MnSOD, is targeted to mitochondria and is instrumental in regulating ROS by conversion of superoxides to hydrogen peroxide, which is further broken down into H(2)O and oxygen. Here, we describe the identification of a novel mutation within the mitochondrial SOD2 enzyme in Drosophila that results in adults with an extremely shortened life span, sensitivity to hyperoxia, and neuropathology. Additional studies demonstrate that this novel mutant, SOD2(bewildered), exhibits abnormal brain morphology, suggesting a critical role for this protein in neurodevelopment. We investigated the basis of this neurodevelopmental defect and discovered an increase in aberrant axonal that could underlie the aberrant neurodevelopment and brain morphology defects. This novel allele, SOD2(bewildered), provides a unique opportunity to study the effects of increased mitochondrial ROS on neural development, axonal targeting, and neural cell degeneration in vivo.

Keywords: Drosophila melanogaster; MnSOD; ROS; SOD2; motoneuron axonal targeting; roGFP

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