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Wu RG, Yang CS, Cheing CC, et al. Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells. Interface Focus. 2011;1(5):744-53doi: 10.1098/rsfs.2011.0049.
Wu, R. G., Yang, C. S., Cheing, C. C., & Tseng, F. G. (2011). Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells. Interface focus, 1(5), 744-53. https://doi.org/10.1098/rsfs.2011.0049
Wu, Ren-Guei, et al. "Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells." Interface focus vol. 1,5 (2011): 744-53. doi: https://doi.org/10.1098/rsfs.2011.0049
Wu RG, Yang CS, Cheing CC, Tseng FG. Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells. Interface Focus. 2011 Oct 06;1(5):744-53. doi: 10.1098/rsfs.2011.0049. Epub 2011 Jul 14. PMID: 23050079; PMCID: PMC3262288.
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Interface Focus. 2011 Oct 06;1(5):744-53. doi: 10.1098/rsfs.2011.0049. Epub 2011 Jul 14.

Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells.

Interface focus

Ren-Guei Wu, Chung-Shi Yang, Ching-Chang Cheing, Fan-Gang Tseng

Affiliations

  1. Department of Engineering and Systems Science , National Tsing Hua University , 101 Section 2 Kuang Fu Road, Hsinchu 300, Taiwan , Republic of China ; National Health Research Institutes , 35 Keyan Road, Zhunan, Miaoli 350, Taiwan , Republic of China.

PMID: 23050079 PMCID: PMC3262288 DOI: 10.1098/rsfs.2011.0049

Abstract

A novel nanocapillary electrophoretic electrochemical (Nano-CEEC) chip has been developed to demonstrate the possibility of zeptomole-level detection of neurotransmitters released from single living cells. The chip integrates three subunits to collect and concentrate scarce neurotransmitters released from single PC-12 cells, including a pair of targeting electrodes for single cells captured by controlling the surface charge density; a dual-asymmetry electrokinetic flow device for sample collection, pre-concentration and separation in a nanochannel; and an online electrochemical detector for zeptomole-level sample detection. This Nano-CEEC chip integrates a polydimethylsiloxane microchannel for cell sampling and biomolecule separation and a silicon dioxide nanochannel for sample pre-concentration and amperometric detection. The cell-capture voltage ranges from 0.1 to 1.5 V with a frequency of 1-10 kHz for PC-12 cells, and the single cell-capture efficiency is optimized by varying the duration of the applied field. All of the processes, from cell sampling to neurotransmitter detection, can be completed within 15 min. Catecholamines, including dopamine and norepinephrine (noradrenaline) released from coupled single cells, have been successfully detected using the Nano-CEEC chip. A detection limit of 30-75 zeptomoles was achieved, which is close to the levels released by a single neuron in vitro.

Keywords: electrochemical detection; nanocapillary electrophoretic electrochemical; single cells analysis

References

  1. Integr Biol (Camb). 2009 Oct;1(10):574-86 - PubMed
  2. Electrophoresis. 2010 Jan;31(1):55-64 - PubMed
  3. Chem Eng Sci. 2011 Apr 1;66(7):1508-1522 - PubMed
  4. Anal Bioanal Chem. 2010 Nov;398(6):2493-504 - PubMed
  5. Anal Bioanal Chem. 2008 Jun;391(3):933-42 - PubMed
  6. Anal Chem. 2007 Feb 15;79(4):1720-4 - PubMed
  7. Electrophoresis. 2009 Jul;30(14):2523-31 - PubMed
  8. Electroanalysis. 2005 Jul;17(13):1192-1197 - PubMed
  9. Anal Chem. 2010 Jun 15;82(12):5082-7 - PubMed
  10. Lab Chip. 2007 Jun;7(6):731-9 - PubMed
  11. Trends Biotechnol. 2000 Apr;18(4):151-60 - PubMed
  12. Nature. 2006 Jul 27;442(7101):403-11 - PubMed
  13. Anal Chem. 2009 Mar 15;81(6):2294-302 - PubMed
  14. Lab Chip. 2007 Apr;7(4):423-40 - PubMed
  15. Am J Physiol. 1998 Jun;274(6):C1592-600 - PubMed
  16. Bioelectrochemistry. 2001 Jan;53(1):79-86 - PubMed
  17. J R Soc Interface. 2008 Oct 6;5 Suppl 2:S139-50 - PubMed
  18. Anal Chem. 2010 Jun 15;82(12):4848-64 - PubMed
  19. Trends Biotechnol. 2010 Jun;28(6):281-90 - PubMed
  20. Annu Rev Anal Chem (Palo Alto Calif). 2008;1:191-227 - PubMed
  21. Anal Chem. 2007 Apr 1;79(7):2614-21 - PubMed
  22. J Neural Eng. 2011 Feb;8(1):011001 - PubMed
  23. Lab Chip. 2010 Apr 21;10(8):957-85 - PubMed
  24. Lab Chip. 2004 Feb;4(1):47-52 - PubMed

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