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Ramer LM, van Stolk AP, Inskip JA, et al. Plasticity of TRPV1-Expressing Sensory Neurons Mediating Autonomic Dysreflexia Following Spinal Cord Injury. Front Physiol. 2012;3:257doi: 10.3389/fphys.2012.00257.
Ramer, L. M., van Stolk, A. P., Inskip, J. A., Ramer, M. S., & Krassioukov, A. V. (2012). Plasticity of TRPV1-Expressing Sensory Neurons Mediating Autonomic Dysreflexia Following Spinal Cord Injury. Frontiers in physiology, 3257. https://doi.org/10.3389/fphys.2012.00257
Ramer, Leanne M, et al. "Plasticity of TRPV1-Expressing Sensory Neurons Mediating Autonomic Dysreflexia Following Spinal Cord Injury." Frontiers in physiology vol. 3 (2012): 257. doi: https://doi.org/10.3389/fphys.2012.00257
Ramer LM, van Stolk AP, Inskip JA, Ramer MS, Krassioukov AV. Plasticity of TRPV1-Expressing Sensory Neurons Mediating Autonomic Dysreflexia Following Spinal Cord Injury. Front Physiol. 2012 Jul 09;3:257. doi: 10.3389/fphys.2012.00257. eCollection 2012. PMID: 22934013; PMCID: PMC3429033.
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Front Physiol. 2012 Jul 09;3:257. doi: 10.3389/fphys.2012.00257. eCollection 2012.

Plasticity of TRPV1-Expressing Sensory Neurons Mediating Autonomic Dysreflexia Following Spinal Cord Injury.

Frontiers in physiology

Leanne M Ramer, A Peter van Stolk, Jessica A Inskip, Matt S Ramer, Andrei V Krassioukov

Affiliations

  1. International Collaboration On Repair Discoveries, University of British Columbia Vancouver, BC, Canada.

PMID: 22934013 PMCID: PMC3429033 DOI: 10.3389/fphys.2012.00257

Abstract

Spinal cord injury (SCI) triggers profound changes in visceral and somatic targets of sensory neurons below the level of injury. Despite this, little is known about the influence of injury to the spinal cord on sensory ganglia. One of the defining characteristics of sensory neurons is the size of their cell body: for example, nociceptors are smaller in size than mechanoreceptors or proprioceptors. In these experiments, we first used a comprehensive immunohistochemical approach to characterize the size distribution of sensory neurons after high- and low-thoracic SCI. Male Wistar rats (300 g) received a spinal cord transection (T3 or T10) or sham-injury. At 30 days post-injury, dorsal root ganglia (DRGs) and spinal cords were harvested and analyzed immunohistochemically. In a wide survey of primary afferents, only those expressing the capsaicin receptor (TRPV1) exhibited somal hypertrophy after T3 SCI. Hypertrophy only occurred caudal to SCI and was pronounced in ganglia far distal to SCI (i.e., in L4-S1 DRGs). Injury-induced hypertrophy was accompanied by a small expansion of central territory in the lumbar spinal dorsal horn and by evidence of TRPV1 upregulation. Importantly, hypertrophy of TRPV1-positive neurons was modest after T10 SCI. Given the specific effects of T3 SCI on TRPV1-positive afferents, we hypothesized that these afferents contribute to autonomic dysreflexia (AD). Rats with T3 SCI received vehicle or capsaicin via intrathecal injection at 2 or 28 days post-SCI; at 30 days, AD was assessed by recording intra-arterial blood pressure during colo-rectal distension (CRD). In both groups of capsaicin-treated animals, the severity of AD was dramatically reduced. While AD is multi-factorial in origin, TRPV1-positive afferents are clearly involved in AD elicited by CRD. These findings implicate TRPV1-positive afferents in the initiation of AD and suggest that TRPV1 may be a therapeutic target for amelioration or prevention of AD after high SCI.

Keywords: capsaicin; colo-rectal distension; dorsal horn; dorsal root; dorsal root ganglion; high blood pressure; hypertension; hypertrophy

References

  1. Exp Neurol. 2005 Mar;192(1):46-59 - PubMed
  2. Am J Physiol Regul Integr Comp Physiol. 2001 May;280(5):R1371-81 - PubMed
  3. J Neurosci. 2004 Dec 1;24(48):10796-805 - PubMed
  4. Exp Neurol. 1997 Oct;147(2):463-75 - PubMed
  5. J Comp Neurol. 2002 Jul 29;449(3):217-30 - PubMed
  6. Exp Neurol. 2003 Nov;184(1):373-80 - PubMed
  7. Neuroscience. 1990;35(2):375-475 - PubMed
  8. Adv Exp Med Biol. 2011;704:861-79 - PubMed
  9. Neuroreport. 2004 Aug 6;15(11):1783-6 - PubMed
  10. Neurochem Int. 2008 May;52(6):1068-75 - PubMed
  11. Am J Physiol Heart Circ Physiol. 2012 Feb 1;302(3):H752-62 - PubMed
  12. J Neurosci. 2006 Oct 18;26(42):10847-55 - PubMed
  13. J Physiol. 2005 Aug 15;567(Pt 1):267-81 - PubMed
  14. Atherosclerosis. 2009 Jan;202(1):29-31 - PubMed
  15. Eur J Neurosci. 2004 Nov;20(10):2567-79 - PubMed
  16. Nature. 1984 Oct 4-10;311(5985):469-72 - PubMed
  17. Spinal Cord. 1999 Jan;37(1):6-13 - PubMed
  18. Neuron. 2005 Sep 15;47(6):787-93 - PubMed
  19. J Neurosci. 2006 Mar 15;26(11):2923-32 - PubMed
  20. Eur J Neurosci. 1997 Aug;9(8):1574-85 - PubMed
  21. J Neurosci. 2006 Aug 16;26(33):8578-87 - PubMed
  22. J Neurobiol. 1997 Jan;32(1):22-32 - PubMed
  23. J Neurotrauma. 2010 Sep;27(9):1709-22 - PubMed
  24. J Neurosci. 1998 Apr 15;18(8):3059-72 - PubMed
  25. Neuron. 2002 Jul 18;35(2):267-82 - PubMed
  26. Neuron. 1998 Dec;21(6):1291-302 - PubMed
  27. J Comp Neurol. 1984 Nov 20;230(1):110-8 - PubMed
  28. Spine (Phila Pa 1976). 2006 Nov 15;31(24):2778-82 - PubMed
  29. J Exp Zool. 1949 Aug;111(3):457-501 - PubMed
  30. Hypertension. 2001 Sep;38(3 Pt 2):581-7 - PubMed
  31. Prog Brain Res. 2006;152:223-9 - PubMed
  32. J Neurosci. 1984 Dec;4(12):2986-92 - PubMed
  33. J Comp Neurol. 1981 Sep 20;201(3):415-40 - PubMed
  34. J Physiol. 1999 Jul 1;518(Pt 1):271-82 - PubMed
  35. J Peripher Nerv Syst. 2006 Dec;11(4):330-45 - PubMed
  36. J Hypertens. 2007 Aug;25(8):1698-703 - PubMed
  37. Curr Hypertens Rep. 2010 Aug;12(4):226-32 - PubMed
  38. J Comp Neurol. 1988 Jun 22;272(4):365-86 - PubMed
  39. Neurourol Urodyn. 2008;27(4):264-73 - PubMed
  40. Brain Res Rev. 2007 Oct;55(2):297-313 - PubMed
  41. Neuroscience. 1993 Dec;57(3):725-32 - PubMed
  42. Neuron. 1998 Sep;21(3):531-43 - PubMed
  43. Am J Physiol Renal Physiol. 2011 Jan;300(1):F49-59 - PubMed
  44. Neuroscience. 2009 Mar 3;159(1):369-79 - PubMed
  45. J Neurotrauma. 2010 Nov;27(11):2077-89 - PubMed
  46. J Neurochem. 2001 May;77(3):864-75 - PubMed
  47. Nature. 1997 Oct 23;389(6653):816-24 - PubMed
  48. Eur J Neurosci. 2003 Nov;18(10):2713-21 - PubMed
  49. Ann N Y Acad Sci. 2010 Nov;1211:66-84 - PubMed
  50. J Auton Nerv Syst. 1995 Sep 5;54(3):215-24 - PubMed
  51. Eur J Neurosci. 2005 Jan;21(1):81-92 - PubMed
  52. Neuroscience. 1998 Jul;85(2):443-58 - PubMed
  53. Proc Natl Acad Sci U S A. 1954 Oct;40(10):1014-8 - PubMed
  54. Neuroscience. 2005;130(1):223-8 - PubMed
  55. J Urol. 2002 Nov;168(5):2269-74 - PubMed
  56. Auton Neurosci. 2006 Apr 30;125(1-2):70-5 - PubMed
  57. Science. 1979 Oct 26;206(4417):481-3 - PubMed
  58. Curr Top Med Chem. 2011;11(17):2118-30 - PubMed
  59. Prog Neurobiol. 1999 Apr;57(6):583-606 - PubMed
  60. Exp Neurol. 2007 Sep;207(1):75-84 - PubMed
  61. Spine J. 2010 Dec;10(12):1108-17 - PubMed
  62. J Comp Neurol. 2003 Oct 6;465(1):121-35 - PubMed
  63. Am J Physiol. 1995 May;268(5 Pt 2):H2077-83 - PubMed
  64. Arch Phys Med Rehabil. 2004 Nov;85(11):1757-63 - PubMed
  65. Am J Physiol Heart Circ Physiol. 2006 Jan;290(1):H398-405 - PubMed
  66. J Neurotrauma. 2001 Oct;18(10):1107-19 - PubMed
  67. J Pharm Pharmacol. 2008 Jun;60(6):717-22 - PubMed
  68. Nature. 1985 Apr 25-May 1;314(6013):751-2 - PubMed
  69. Prog Brain Res. 2009;175:125-37 - PubMed
  70. J Neurosci. 2011 Jul 20;31(29):10516-28 - PubMed
  71. Exp Neurol. 2008 Dec;214(2):301-8 - PubMed
  72. Am J Physiol Regul Integr Comp Physiol. 2011 Jun;300(6):R1392-400 - PubMed
  73. Am J Physiol Regul Integr Comp Physiol. 2010 May;298(5):R1358-65 - PubMed
  74. Nature. 1999 Apr 1;398(6726):436-41 - PubMed
  75. J Neurosci. 1999 Sep 1;19(17):7405-14 - PubMed
  76. J Neurosci. 1991 Feb;11(2):543-52 - PubMed
  77. Spinal Cord. 2009 Sep;47(9):651-61 - PubMed
  78. Prog Brain Res. 2006;152:345-58 - PubMed
  79. Curr Top Med Chem. 2011;11(17):2159-70 - PubMed
  80. J Neurosci. 2010 Nov 3;30(44):14870-82 - PubMed
  81. J Comp Neurol. 1984 Jun 20;226(2):238-45 - PubMed
  82. J Hypertens. 2009 Apr;27(4):838-45 - PubMed
  83. J Neurotrauma. 2011 May;28(5):821-9 - PubMed
  84. J Neurosci. 1999 Mar 1;19(5):1844-54 - PubMed
  85. Prog Brain Res. 2006;152:289-97 - PubMed
  86. Nat Rev Urol. 2011 Oct 04;8(11):617-30 - PubMed

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