Display options
Cite
Ceuppens S, Uyttendaele M, Hamelink S, et al. Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. Gut Pathog. 2012;4(1):11doi: 10.1186/1757-4749-4-11.
Ceuppens, S., Uyttendaele, M., Hamelink, S., Boon, N., & Van de Wiele, T. (2012). Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. Gut pathogens, 4(1), 11. https://doi.org/10.1186/1757-4749-4-11
Ceuppens, Siele, et al. "Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit." Gut pathogens vol. 4,1 (2012): 11. doi: https://doi.org/10.1186/1757-4749-4-11
Ceuppens S, Uyttendaele M, Hamelink S, Boon N, Van de Wiele T. Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit. Gut Pathog. 2012 Oct 03;4(1):11. doi: 10.1186/1757-4749-4-11. PMID: 23034184; PMCID: PMC3500208.
Copy Download .nbib Format: NLM
Share it on

Gut Pathog. 2012 Oct 03;4(1):11. doi: 10.1186/1757-4749-4-11.

Inactivation of Bacillus cereus vegetative cells by gastric acid and bile during in vitro gastrointestinal transit.

Gut pathogens

Siele Ceuppens, Mieke Uyttendaele, Stefanie Hamelink, Nico Boon, Tom Van de Wiele

Affiliations

  1. Faculty of Bioscience Engineering, Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000, Gent, Belgium. [email protected].

PMID: 23034184 PMCID: PMC3500208 DOI: 10.1186/1757-4749-4-11

Abstract

BACKGROUND: The foodborne pathogen Bacillus cereus can cause diarrhoeal food poisoning by production of enterotoxins in the small intestine. The prerequisite for diarrhoeal disease is thus survival during gastrointestinal passage.

METHODS: Vegetative cells of 3 different B. cereus strains were cultivated in a real composite food matrix, lasagne verde, and their survival during subsequent simulation of gastrointestinal passage was assessed using in vitro experiments simulating transit through the human upper gastrointestinal tract (from mouth to small intestine).

RESULTS: No survival of vegetative cells was observed, despite the high inoculum levels of 7.0 to 8.0 log CFU/g and the presence of various potentially protective food components. Significant fractions (approx. 10% of the consumed inoculum) of B. cereus vegetative cells survived gastric passage, but they were subsequently inactivated by bile exposure in weakly acidic intestinal medium (pH 5.0). In contrast, the low numbers of spores present (up to 4.0 log spores/g) showed excellent survival and remained viable spores throughout the gastrointestinal passage simulation.

CONCLUSION: Vegetative cells are inactivated by gastric acid and bile during gastrointestinal passage, while spores are resistant and survive. Therefore, the physiological form (vegetative cells or spores) of the B. cereus consumed determines the subsequent gastrointestinal survival and thus the infective dose, which is expected to be much lower for spores than vegetative cells. No significant differences in gastrointestinal survival ability was found among the different strains. However, considerable strain variability was observed in sporulation tendency during growth in laboratory medium and food, which has important implications for the gastrointestinal survival potential of the different B. cereus strains.

References

  1. Appl Environ Microbiol. 2002 Dec;68(12):6005-12 - PubMed
  2. J Exp Biol. 2001 May;204(Pt 10):1673-86 - PubMed
  3. Food Microbiol. 2011 May;28(3):364-72 - PubMed
  4. Eur J Gastroenterol Hepatol. 1997 Jul;9(7):703-9 - PubMed
  5. J Lipid Res. 1992 May;33(5):617-26 - PubMed
  6. Dig Dis Sci. 1990 May;35(5):589-95 - PubMed
  7. Crit Rev Microbiol. 2011 Aug;37(3):188-213 - PubMed
  8. Appl Environ Microbiol. 2012 Nov;78(21):7698-705 - PubMed
  9. J Chromatogr. 1985 Nov 27;348(1):151-7 - PubMed
  10. Foodborne Pathog Dis. 2012 Dec;9(12):1130-6 - PubMed
  11. Hepatology. 1990 Sep;12(3 Pt 2):6S-14S; discussion 14S-16S - PubMed
  12. J Food Prot. 2012 Apr;75(4):690-4 - PubMed
  13. J Appl Bacteriol. 1993 Jan;74(1):36-40 - PubMed
  14. Int J Food Microbiol. 2000 Nov 1;61(2-3):193-7 - PubMed
  15. Gut. 1973 Jul;14(7):513-8 - PubMed
  16. Int J Food Microbiol. 2003 Mar 15;81(2):131-6 - PubMed
  17. J Appl Microbiol. 2007 Nov;103(5):1568-75 - PubMed
  18. Appl Environ Microbiol. 1996 Jul;62(7):2416-20 - PubMed
  19. Int J Food Microbiol. 1999 Apr 1;48(1):21-35 - PubMed
  20. Int J Food Microbiol. 2012 Apr 16;155(3):241-6 - PubMed
  21. Toxicology. 2004 Oct 15;203(1-3):1-15 - PubMed
  22. FEMS Microbiol Rev. 2005 Sep;29(4):625-51 - PubMed
  23. J Bacteriol. 1998 Sep;180(17):4686-92 - PubMed
  24. Proc Soc Exp Biol Med. 1959 Apr;100(4):676-8 - PubMed
  25. Int J Food Microbiol. 2003 Apr 25;82(2):191-8 - PubMed
  26. Hepatology. 1998 Feb;27(2):615-20 - PubMed
  27. Dig Dis. 1998 Mar-Apr;16(2):118-24 - PubMed
  28. J Dairy Sci. 1993 May;76(5):1253-9 - PubMed
  29. Int J Food Microbiol. 2006 Nov 1;112(2):120-8 - PubMed
  30. J Bacteriol. 2007 Jul;189(14):5302-13 - PubMed
  31. Infect Immun. 2000 Dec;68(12):6763-9 - PubMed
  32. Z Gastroenterol. 1996 Jun;34(6):365-70 - PubMed

Publication Types