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Baker ES, Liu T, Petyuk VA, et al. Mass spectrometry for translational proteomics: progress and clinical implications. Genome Med. 2012;4(8):63doi: 10.1186/gm364.
Baker, E. S., Liu, T., Petyuk, V. A., Burnum-Johnson, K. E., Ibrahim, Y. M., Anderson, G. A., & Smith, R. D. (2012). Mass spectrometry for translational proteomics: progress and clinical implications. Genome medicine, 4(8), 63. https://doi.org/10.1186/gm364
Baker, Erin Shammel, et al. "Mass spectrometry for translational proteomics: progress and clinical implications." Genome medicine vol. 4,8 (2012): 63. doi: https://doi.org/10.1186/gm364
Baker ES, Liu T, Petyuk VA, Burnum-Johnson KE, Ibrahim YM, Anderson GA, Smith RD. Mass spectrometry for translational proteomics: progress and clinical implications. Genome Med. 2012 Aug 31;4(8):63. doi: 10.1186/gm364. eCollection 2012. PMID: 22943415; PMCID: PMC3580401.
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Genome Med. 2012 Aug 31;4(8):63. doi: 10.1186/gm364. eCollection 2012.

Mass spectrometry for translational proteomics: progress and clinical implications.

Genome medicine

Erin Shammel Baker, Tao Liu, Vladislav A Petyuk, Kristin E Burnum-Johnson, Yehia M Ibrahim, Gordon A Anderson, Richard D Smith

Affiliations

  1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

PMID: 22943415 PMCID: PMC3580401 DOI: 10.1186/gm364

Abstract

The utility of mass spectrometry (MS)-based proteomic analyses and their clinical applications have been increasingly recognized over the past decade due to their high sensitivity, specificity and throughput. MS-based proteomic measurements have been used in a wide range of biological and biomedical investigations, including analysis of cellular responses and disease-specific post-translational modifications. These studies greatly enhance our understanding of the complex and dynamic nature of the proteome in biology and disease. Some MS techniques, such as those for targeted analysis, are being successfully applied for biomarker verification, whereas others, including global quantitative analysis (for example, for biomarker discovery), are more challenging and require further development. However, recent technological improvements in sample processing, instrumental platforms, data acquisition approaches and informatics capabilities continue to advance MS-based applications. Improving the detection of significant changes in proteins through these advances shows great promise for the discovery of improved biomarker candidates that can be verified pre-clinically using targeted measurements, and ultimately used in clinical studies - for example, for early disease diagnosis or as targets for drug development and therapeutic intervention. Here, we review the current state of MS-based proteomics with regard to its advantages and current limitations, and we highlight its translational applications in studies of protein biomarkers.

Keywords: biomarker; clinical proteomics; ion mobility separations; mass spectrometry; multiple reaction monitoring; selected reaction monitoring; shotgun proteomics; targeted proteomics; translational proteomics

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