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Chen YF, Ching C, Wu TS, et al. Balanophora spicata and Lupeol Acetate Possess Antinociceptive and Anti-Inflammatory Activities In Vivo and In Vitro. Evid Based Complement Alternat Med. 2012;2012:371273doi: 10.1155/2012/371273.
Chen, Y. F., Ching, C., Wu, T. S., Wu, C. R., Hsieh, W. T., & Tsai, H. Y. (2012). Balanophora spicata and Lupeol Acetate Possess Antinociceptive and Anti-Inflammatory Activities In Vivo and In Vitro. Evidence-based complementary and alternative medicine : eCAM, 2012371273. https://doi.org/10.1155/2012/371273
Chen, Yuh-Fung, et al. "Balanophora spicata and Lupeol Acetate Possess Antinociceptive and Anti-Inflammatory Activities In Vivo and In Vitro." Evidence-based complementary and alternative medicine : eCAM vol. 2012 (2012): 371273. doi: https://doi.org/10.1155/2012/371273
Chen YF, Ching C, Wu TS, Wu CR, Hsieh WT, Tsai HY. Balanophora spicata and Lupeol Acetate Possess Antinociceptive and Anti-Inflammatory Activities In Vivo and In Vitro. Evid Based Complement Alternat Med. 2012;2012:371273. doi: 10.1155/2012/371273. Epub 2012 Nov 11. PMID: 23243439; PMCID: PMC3518255.
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Evid Based Complement Alternat Med. 2012;2012:371273. doi: 10.1155/2012/371273. Epub 2012 Nov 11.

Balanophora spicata and Lupeol Acetate Possess Antinociceptive and Anti-Inflammatory Activities In Vivo and In Vitro.

Evidence-based complementary and alternative medicine : eCAM

Yuh-Fung Chen, Chien Ching, Tian-Shung Wu, Chi-Rei Wu, Wen-Tsong Hsieh, Huei-Yann Tsai

Affiliations

  1. Department of Pharmacology, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Department of Pharmacy, China Medical University Hospital, Taichung 40427, Taiwan.

PMID: 23243439 PMCID: PMC3518255 DOI: 10.1155/2012/371273

Abstract

Aims of the present study were to investigate effects of Balanophora spicata (BS) on antinociception and anti-inflammation both in vivo and in vitro. Crude extract of BS inhibited vascular permeability induced by histamine, serotonin, bradykinin, and PGE(2), but not by PAF. Furthermore, BS crude extract, different layers (n-hexane, ethyl acetate, n-butanol, and water layer), and lupeol acetate had significant antinociceptive and anti-inflammatory effects on acetic acid-induced abdominal writhing response, formalin-induced licking behavior, carrageenan-, and serotonin-induced paw edema. The n-hexane layer had the most effective potency among all layers (IC50: 67.33 mg/kg on writhing response; IC50s: 34.2 mg/kg and 21.29 mg/kg on the early phase and late phase of formalin test, resp.). Additionally, lupeol acetate which was isolated from the n-hexane layer of BS effectively inhibited the acetic acid-induced writhing response (IC50: 28.32 mg/kg), formalin-induced licking behavior (IC50: 20.95 mg/kg), NO production (IC50: 4.102 μM), iNOS expression (IC50: 5.35 μM), and COX2 expression (IC50: 5.13 μM) in LPS-stimulated RAW 264.7 cells. In conclusion, BS has antinociceptive and anti-inflammatory effects which may be partially due to the inhibition of changes in vascular permeability induced by histamine, serotonin, bradykinin, and PGE(2) and the attenuation of iNOS and COX-2 expression.

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