Display options
Cite
Nielsen KH, Staley JP. Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!. Genes Dev. 2012;26(22):2461-7doi: 10.1101/gad.207514.112.
Nielsen, K. H., & Staley, J. P. (2012). Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!. Genes & development, 26(22), 2461-7. https://doi.org/10.1101/gad.207514.112
Nielsen, Klaus H, and Staley, Jonathan P. "Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!." Genes & development vol. 26,22 (2012): 2461-7. doi: https://doi.org/10.1101/gad.207514.112
Nielsen KH, Staley JP. Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!. Genes Dev. 2012 Nov 15;26(22):2461-7. doi: 10.1101/gad.207514.112. PMID: 23154979; PMCID: PMC3505815.
Copy Download .nbib Format: NLM
Share it on

Genes Dev. 2012 Nov 15;26(22):2461-7. doi: 10.1101/gad.207514.112.

Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!.

Genes & development

Klaus H Nielsen, Jonathan P Staley

Affiliations

  1. Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.

PMID: 23154979 PMCID: PMC3505815 DOI: 10.1101/gad.207514.112

Abstract

During pre-mRNA splicing, the spliceosome is activated for catalysis by unwinding base-paired U4/U6 small nuclear RNAs, a step that must be precisely timed. We know that unwinding requires the ATPase Brr2, but the mechanism and regulation of unwinding have been understood poorly. In the November 1, 2012, issue of Genes & Development, Hahn and colleagues (pp. 2408-2421) and Mozaffari-Jovin and colleagues (pp. 2422-2434) defined a pathway for U4/U6 unwinding. Moreover, Mozaffari-Jovin and colleagues suggested a mechanism for regulating Brr2.

References

  1. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4188-92 - PubMed
  2. Mol Cell. 2006 Feb 17;21(4):543-53 - PubMed
  3. EMBO J. 2002 Oct 15;21(20):5527-38 - PubMed
  4. RNA. 1996 Aug;2(8):835-48 - PubMed
  5. Cell. 1996 Mar 8;84(5):801-11 - PubMed
  6. Nat Struct Mol Biol. 2009 Jul;16(7):731-9 - PubMed
  7. Nature. 2012 Jun 20;486(7403):368-74 - PubMed
  8. Cell. 1992 Nov 27;71(5):803-17 - PubMed
  9. Nat Struct Mol Biol. 2008 May;15(5):444-51 - PubMed
  10. RNA. 2012 May;18(5):1075-90 - PubMed
  11. RNA. 1999 Jul;5(7):959-71 - PubMed
  12. Nucleic Acids Res. 1996 Mar 1;24(5):868-75 - PubMed
  13. EMBO J. 2008 Nov 5;27(21):2929-40 - PubMed
  14. Genes Dev. 2012 Nov 1;26(21):2408-21 - PubMed
  15. Genes Dev. 2012 Nov 1;26(21):2422-34 - PubMed
  16. Genetics. 2000 Aug;155(4):1667-82 - PubMed
  17. Cell. 1996 Aug 23;86(4):679-89 - PubMed
  18. Nat Struct Mol Biol. 2009 Jan;16(1):42-8 - PubMed
  19. Mol Cell Biol. 1992 Apr;12(4):1460-8 - PubMed
  20. Am J Hum Genet. 2009 Nov;85(5):617-27 - PubMed
  21. RNA. 1996 Mar;2(3):213-25 - PubMed
  22. Nat Struct Mol Biol. 2008 Dec;15(12):1235-7 - PubMed
  23. Proc Natl Acad Sci U S A. 2010 Jun 1;107(22):10020-5 - PubMed
  24. Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9145-9 - PubMed
  25. Science. 2001 Jan 5;291(5501):121-5 - PubMed
  26. Methods Enzymol. 2012;511:275-88 - PubMed
  27. Proc Natl Acad Sci U S A. 2012 Oct 23;109(43):17418-23 - PubMed
  28. Biochemistry. 2005 May 31;44(21):7903-11 - PubMed
  29. Mol Cell. 2001 Jan;7(1):217-26 - PubMed
  30. Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):10010-5 - PubMed
  31. Genes Dev. 2010 Jul 1;24(13):1434-47 - PubMed
  32. Nature. 2000 Dec 14;408(6814):881-4 - PubMed
  33. Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):13817-22 - PubMed
  34. Nat Struct Mol Biol. 2008 Nov;15(11):1199-205 - PubMed
  35. Cold Spring Harb Perspect Biol. 2011 Jul 01;3(7): - PubMed
  36. Mol Cell. 2006 Aug 4;23(3):389-99 - PubMed
  37. Nat Struct Mol Biol. 2007 Jul;14(7):647-52 - PubMed
  38. Curr Biol. 1998 Jul 16;8(15):847-55 - PubMed
  39. RNA. 2006 Feb;12(2):292-302 - PubMed
  40. Mol Cell. 2009 Aug 28;35(4):454-66 - PubMed
  41. Genetics. 2005 Jul;170(3):1063-80 - PubMed
  42. RNA. 2009 Jul;15(7):1386-97 - PubMed
  43. RNA. 2006 Jan;12(1):83-93 - PubMed
  44. EMBO Rep. 2002 Sep;3(9):875-80 - PubMed
  45. Nucleic Acids Res. 1997 Jun 1;25(11):2146-52 - PubMed
  46. EMBO Rep. 2010 Mar;11(3):180-6 - PubMed
  47. Curr Opin Cell Biol. 2012 Jun;24(3):431-8 - PubMed

Publication Types

Grant support