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Ashavaid TF, Kumbhat NS. Isolation and characterization of mitochondria from goat hearts. Indian J Clin Biochem. 2005;20(1):72-9doi: 10.1007/BF02893046.
Ashavaid, T. F., & Kumbhat, N. S. (2005). Isolation and characterization of mitochondria from goat hearts. Indian journal of clinical biochemistry : IJCB, 20(1), 72-9. https://doi.org/10.1007/BF02893046
Ashavaid, Tester F, and Kumbhat, Neena S. "Isolation and characterization of mitochondria from goat hearts." Indian journal of clinical biochemistry : IJCB vol. 20,1 (2005): 72-9. doi: https://doi.org/10.1007/BF02893046
Ashavaid TF, Kumbhat NS. Isolation and characterization of mitochondria from goat hearts. Indian J Clin Biochem. 2005 Jan;20(1):72-9. doi: 10.1007/BF02893046. PMID: 23105498; PMCID: PMC3454148.
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Indian J Clin Biochem. 2005 Jan;20(1):72-9. doi: 10.1007/BF02893046.

Isolation and characterization of mitochondria from goat hearts.

Indian journal of clinical biochemistry : IJCB

Tester F Ashavaid, Neena S Kumbhat

Affiliations

  1. Research Laboratories, P.D. Hinduja National Hospital and Medical Research Centre, Veer Savarkar Marg, 400 016 Mahim, Mumbai India.

PMID: 23105498 PMCID: PMC3454148 DOI: 10.1007/BF02893046

Abstract

Cardiac mitochondria provide energy for the contraction/relaxation cycle. The aim of our study was to isolate and characterize mitochondria from Caprine hearts under control and in-vitro induced ischemia. A decrease in activities of all the enzymes was observed in the ischemic models. Further characterization of proteins was done by SDS-PAGE and BN-PAGE. Lipids have been characterized by analyzing the phospholipids by HPTLC and fatty acids by GLC in both groups. Our results indicated that injury occurs early in the course of ischemia and progresses during ischemia. TBARS and carbonyl content have also been measured. The in-vitro effects of fatty acids have been studied on the enzymes and complexes of mitochondria.

Keywords: Heart; ischemia; lipids; marker enzymes; respiratory chain complexes

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