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Norouzi M, Ghorashi S, Abedi F, et al. Identification of Hepatitis B Virus Surface Antigen (HBsAg) Genotypes and Variations in Chronic Carriers from Isfahan Province, Iran. Iran J Public Health. 2012;41(3):104-11
Norouzi, M., Ghorashi, S., Abedi, F., Nejatizadeh, A., Ataei, B., Malekzadeh, R., Alavian, S., Judaki, M., Ghamari, S., Namazi, A., Rahimnia, R., Khedive, A., & Jazayeri, S. (2012). Identification of Hepatitis B Virus Surface Antigen (HBsAg) Genotypes and Variations in Chronic Carriers from Isfahan Province, Iran. Iranian journal of public health, 41(3), 104-11.
Norouzi, M, et al. "Identification of Hepatitis B Virus Surface Antigen (HBsAg) Genotypes and Variations in Chronic Carriers from Isfahan Province, Iran." Iranian journal of public health vol. 41,3 (2012): 104-11.
Norouzi M, Ghorashi S, Abedi F, Nejatizadeh A, Ataei B, Malekzadeh R, Alavian S, Judaki M, Ghamari S, Namazi A, Rahimnia R, Khedive A, Jazayeri S. Identification of Hepatitis B Virus Surface Antigen (HBsAg) Genotypes and Variations in Chronic Carriers from Isfahan Province, Iran. Iran J Public Health. 2012;41(3):104-11. Epub 2012 Mar 31. PMID: 23113154; PMCID: PMC3481712.
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Iran J Public Health. 2012;41(3):104-11. Epub 2012 Mar 31.

Identification of Hepatitis B Virus Surface Antigen (HBsAg) Genotypes and Variations in Chronic Carriers from Isfahan Province, Iran.

Iranian journal of public health

M Norouzi, Sa Ghorashi, F Abedi, A Nejatizadeh, B Ataei, R Malekzadeh, Sm Alavian, Ma Judaki, S Ghamari, A Namazi, R Rahimnia, A Khedive, Sm Jazayeri

Affiliations

  1. Iranian National Institute for Genetic Engineering and Biotechnology, Tehran, Iran.

PMID: 23113154 PMCID: PMC3481712

Abstract

BACKGROUND: Hepatitis B virus (HBV) gene and protein variations are frequently been seen in chronic patients. The aims of study were to determine the genotypes as well as the patterns of variations distribution in chronically-infected patients from the central part of Iran.

METHODS: The surface gene was amplified, sequenced and subsequently aligned using international and national Iranian database.

RESULTS: All strains belonged to genotype D, subgenotype D1 and subtype ayw2. Of all 62 mutations occurred at 39 nucleotide positions, 31 (50%) were missense (amino acid altering) and 31 (50%) were silent (no amino acid changing). At the amino acid level, 30 substitutions occurred, however, 3 were in positions 122 and 127, corresponded to subtypic determination. 22 (73%) out of 30 amino acid mutations occurred in different immune epitopes within surface protein, of which 12 (54.54%) in B cell epitopes in 10 residues; 5 (45.45%) in T helper epitopes in positions; 5 (22.73%) in inside CTL epitopes in 4 residues.

CONCLUSION: The distribution of amino acid mutations as well as the ratio between silent and missense nucleotide mutations showed a narrowly focused immune pressure had already been on the surface protein in these patients, led to the emergence of escape mutants in these patients.

Keywords: HBV genotype D; HBV genotype in Iran; HBV genotypes; HBV immune epitopes

References

  1. Jpn J Exp Med. 1987 Aug;57(4):231-6 - PubMed
  2. J Gen Virol. 1993 Aug;74 ( Pt 8):1627-32 - PubMed
  3. J Gastrointestin Liver Dis. 2007 Dec;16(4):403-6 - PubMed
  4. J Gen Virol. 2000 Jan;81(Pt 1):67-74 - PubMed
  5. Vaccine. 2000 Feb 18;18 Suppl 1:S20-2 - PubMed
  6. Virology. 1994 Feb;198(2):489-503 - PubMed
  7. Mol Biol Evol. 2007 Aug;24(8):1596-9 - PubMed
  8. Springer Semin Immunopathol. 1995;17(2-3):261-81 - PubMed
  9. J Gen Virol. 2002 Aug;83(Pt 8):2059-2073 - PubMed
  10. J Viral Hepat. 2004 Nov;11(6):488-501 - PubMed

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