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Sgarbi MW, Silva BA, Peres CM, et al. Leukocyte infiltration in lung, muscle, and liver after limb compression in rats. Pathophysiology. 2013;20(2):111-6doi: 10.1016/j.pathophys.2012.12.001.
Sgarbi, M. W., Silva, B. A., Peres, C. M., Alba, T. C., Curi, R., Soriano, F. G., Ribeiro, D. A., & Velasco, I. T. (2013). Leukocyte infiltration in lung, muscle, and liver after limb compression in rats. Pathophysiology : the official journal of the International Society for Pathophysiology, 20(2), 111-6. https://doi.org/10.1016/j.pathophys.2012.12.001
Sgarbi, Mauricio Wanderley Moral, et al. "Leukocyte infiltration in lung, muscle, and liver after limb compression in rats." Pathophysiology : the official journal of the International Society for Pathophysiology vol. 20,2 (2013): 111-6. doi: https://doi.org/10.1016/j.pathophys.2012.12.001
Sgarbi MW, Silva BA, Peres CM, Alba TC, Curi R, Soriano FG, Ribeiro DA, Velasco IT. Leukocyte infiltration in lung, muscle, and liver after limb compression in rats. Pathophysiology. 2013 Apr;20(2):111-6. doi: 10.1016/j.pathophys.2012.12.001. Epub 2013 Feb 15. PMID: 23415541.
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Pathophysiology. 2013 Apr;20(2):111-6. doi: 10.1016/j.pathophys.2012.12.001. Epub 2013 Feb 15.

Leukocyte infiltration in lung, muscle, and liver after limb compression in rats.

Pathophysiology : the official journal of the International Society for Pathophysiology

Mauricio Wanderley Moral Sgarbi, Bomfim A Silva, Carmem Maldonado Peres, Tatiana Carolina Alba, Rui Curi, Francisco Garcia Soriano, Daniel Araki Ribeiro, Irineu Tadeu Velasco

Affiliations

  1. School of Medicine, University of São Paulo (USP), SP, Brazil; Department of Biosciences, Federal University of São Paulo, UNIFESP, SP, Brazil. Electronic address: [email protected].

PMID: 23415541 DOI: 10.1016/j.pathophys.2012.12.001

Abstract

Muscle crush injury is associated with systemic manifestations known as crush syndrome. A systemic inflammatory response syndrome may be triggered by isolated crush injury. Using myeloperoxidase (MPO) activity and plasma fatty acid composition, we investigated the inflammatory response in distant organs after isolated limb compression in rats. Male Wistar rats were submitted to 1h of hind limb compression by a latex ribbon. Myeloperoxidase activity was measured in muscle, liver, and lung at progressive times (1, 2 or 4h) after bandage release. Plasma fatty acid composition was evaluated as an indirect measure of oxidative stress. The liver and hind limb muscles showed a transient increase in MPO activity. Pulmonary MPO activity, otherwise, increased progressively throughout the study and reached statistically significant values at 4h when compared to all other groups (p<0.05). Plasma levels of unsaturated fatty acids decreased gradually after decompression (p<0.05 compared to controls after 4h). Blunt traumatic muscle compression was associated with rapid and transient muscle and liver inflammatory cell infiltration but otherwise, polymorphonuclear cells showed progressive aggregation in lungs. The plasmatic unsaturated index decreased throughout the 4h after muscle release. We demonstrated that limb compression was associated with oxidative stress and distant inflammatory responses. Progressive inflammatory cell infiltration in lungs could be related with the delayed systemic adverse responses found after crush injury.

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