Display options
Cite
Sanchez JA, Spina ML, Perera OP. Analysis of the population structure of Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) in the Palaearctic region using microsatellite markers. Ecol Evol. 2012;2(12):3145-59doi: 10.1002/ece3.420.
Sanchez, J. A., Spina, M. L., & Perera, O. P. (2012). Analysis of the population structure of Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) in the Palaearctic region using microsatellite markers. Ecology and evolution, 2(12), 3145-59. https://doi.org/10.1002/ece3.420
Sanchez, Juan Antonio, et al. "Analysis of the population structure of Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) in the Palaearctic region using microsatellite markers." Ecology and evolution vol. 2,12 (2012): 3145-59. doi: https://doi.org/10.1002/ece3.420
Sanchez JA, Spina ML, Perera OP. Analysis of the population structure of Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) in the Palaearctic region using microsatellite markers. Ecol Evol. 2012 Dec;2(12):3145-59. doi: 10.1002/ece3.420. Epub 2012 Nov 08. PMID: 23301179; PMCID: PMC3539007.
Copy Download .nbib Format: NLM
Share it on

Ecol Evol. 2012 Dec;2(12):3145-59. doi: 10.1002/ece3.420. Epub 2012 Nov 08.

Analysis of the population structure of Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) in the Palaearctic region using microsatellite markers.

Ecology and evolution

Juan Antonio Sanchez, Michelangelo La Spina, Omaththage P Perera

Affiliations

  1. Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA) C/Mayor, 1, La Alberca, 30150, Murcia, Spain.

PMID: 23301179 PMCID: PMC3539007 DOI: 10.1002/ece3.420

Abstract

Macrolophus pygmaeus (Rambur) (Hemiptera: Miridae) is widely distributed throughout the Palaearctic region. The aim was to explain the current geographic distribution of the species by investigating its genetic population structure. Samples of M. pygmaeus were collected in 15 localities through its range of distribution. A sample from a commercial producer was also analyzed. A total of 367 M. pygmaeus were genotyped for nine microsatellite loci. Isolation by distance was tested by Mantel's test. The molecular structure of M. pygmaeus populations was inferred by UPGMA, AMOVA, Principal component and Bayesian analyses. The average number of alleles per locus per population was 5.5 (range: 3.1-7.8). Istanbul (Turkey) and Nimes (France) had the lowest (0.291) and the highest (0.626) expected heterozygosity (H(e)), respectively. There was an increase in H(e) from the Canary Islands to Nimes, and a progressive decrease thereafter. A significant negative correlation was found between allelic richness and H(e), and the distance of each population to the easternmost locality (Canary Islands). Significant linkage disequilibrium was observed in the populations from Turkey. F(ST) (0.004-0.334) indicated a high population differentiation, with isolation by distance supported by a high correlation. Bayesian analyses, PCA, and UPGMA pointed to three main clusters: (1) Greece and Turkey, (2) Italy and France, and (3) southern Iberia and the Canary Islands. The recent evolutionary history of M. pygmaeus is inferred from the data as follows: (1) the reduction in the geographic distribution of the species to the Iberian, Italian, and Balkan peninsulas, and possibly southern France, during glaciations and re-colonization of northern Europe from its southern refuges; (2) the maintenance of high diversity in Iberia and Italy (and possibly southern France) during contraction periods, and bottlenecks in the Balkans; (3) introgression of the Italian-French lineage in northern Spain, naturally or through trade.

Keywords: Evolutionary history; Mediterranean peninsulas; geographic distribution; mirids; molecular diversity; plant bugs

References

  1. Bioinformatics. 2012 Oct 1;28(19):2537-9 - PubMed
  2. Nature. 2000 Jun 22;405(6789):907-13 - PubMed
  3. Heredity (Edinb). 2009 Sep;103(3):238-47 - PubMed
  4. Mol Ecol. 2005 Jul;14(8):2611-20 - PubMed
  5. Mol Ecol. 1998 Apr;7(4):453-64 - PubMed
  6. Plant Mol Biol. 2005 Mar;57(4):461-85 - PubMed
  7. Mol Ecol. 1995 Feb;4(1):49-60 - PubMed
  8. Heredity (Edinb). 2009 Apr;102(4):402-12 - PubMed
  9. Mol Biol Evol. 2007 Mar;24(3):621-31 - PubMed
  10. Evolution. 1996 Dec;50(6):2515-2520 - PubMed
  11. Genetics. 1996 Sep;144(1):389-99 - PubMed
  12. Genetica. 2005 Nov;125(2-3):231-41 - PubMed
  13. Am J Hum Genet. 1967 May;19(3 Pt 1):233-57 - PubMed
  14. Bioinformatics. 2008 Jun 1;24(11):1403-5 - PubMed
  15. Mol Ecol. 2006 May;15(6):1603-15 - PubMed
  16. Comput Appl Biosci. 1996 Aug;12(4):357-8 - PubMed
  17. Trends Genet. 2004 Feb;20(2):103-11 - PubMed
  18. Mol Ecol Resour. 2008 Jan;8(1):103-6 - PubMed
  19. Mol Ecol Resour. 2008 May;8(3):690-1 - PubMed
  20. Evol Bioinform Online. 2007 Feb 23;1:47-50 - PubMed
  21. Science. 1996 Mar 8;271(5254):1380-7 - PubMed
  22. Heredity (Edinb). 2011 Apr;106(4):625-32 - PubMed
  23. Bull Entomol Res. 2003 Dec;93(6):507-14 - PubMed
  24. Evolution. 1989 Jan;43(1):223-225 - PubMed
  25. Mol Ecol Resour. 2011 May;11(3):586-9 - PubMed
  26. Mol Ecol. 2008 Oct;17(20):4470-9 - PubMed
  27. Genetics. 2000 Jun;155(2):945-59 - PubMed
  28. Evolution. 1984 Nov;38(6):1358-1370 - PubMed
  29. Med Vet Entomol. 2009 Mar;23(1):69-77 - PubMed
  30. Front Zool. 2007 Apr 17;4:11 - PubMed
  31. Ecol Lett. 2006 May;9(5):615-29 - PubMed
  32. J Evol Biol. 2011 Jan;24(1):146-58 - PubMed

Publication Types