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Dhamodharan R, Hoti S, Sivapragasam G, et al. Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti. Trop Parasitol. 2011;1(2):76-82doi: 10.4103/2229-5070.86935.
Dhamodharan, R., Hoti, S., Sivapragasam, G., & Das, M. (2011). Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti. Tropical parasitology, 1(2), 76-82. https://doi.org/10.4103/2229-5070.86935
Dhamodharan, R, et al. "Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti." Tropical parasitology vol. 1,2 (2011): 76-82. doi: https://doi.org/10.4103/2229-5070.86935
Dhamodharan R, Hoti S, Sivapragasam G, Das M. Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti. Trop Parasitol. 2011 Jul;1(2):76-82. doi: 10.4103/2229-5070.86935. PMID: 23508470; PMCID: PMC3593480.
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Trop Parasitol. 2011 Jul;1(2):76-82. doi: 10.4103/2229-5070.86935.

Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti.

Tropical parasitology

R Dhamodharan, Sl Hoti, G Sivapragasam, Mk Das

Affiliations

  1. Microbiology and Molecular Biology Division, Vector Control Research Centre (ICMR), Puducherry, India.

PMID: 23508470 PMCID: PMC3593480 DOI: 10.4103/2229-5070.86935

Abstract

BACKGROUND: Periplasmic serine proteases of HtrA type of Wolbachia have been shown to play a role in the pathogenesis of filarial disease.

AIMS: This study was aimed to sequence Wb-HtrA serine protease and analyze its phylogenetic position by comparing with other filarial and non-filarial nematode homologs.

MATERIALS AND METHODS: Partial HtrA gene fragment was amplified from DNA isolated from periodic and sub-periodic Wuchereria bancrofti parasites collected from Pondicherry and Nicobar islands, respectively. The amplicons were sequenced, and sequence homology and phylogenetic relationship with other filarial and non-filarial nematodes were analyzed.

RESULTS: Partial orthologue of HtrA-type serine protease from Wolbachia of W. bancrofti was amplified, cloned and sequenced. The deduced amino acid sequence exhibited 87%, 81% and 74% identity with the homologous Wolbachia proteases identified from Brugia malayi, Onchocerca volvulus and Drosophila melanogaster, respectively. The Wb-HtrA has arthologues in several proteobacteria with very high homology and hence is highly conserved not only among Wolbachia of filarial parasites but also across proteobacteria. The phylogenetic tree constructed using Neighbor-Joining method showed two main clusters: cluster-I containing bacteria that dwell in diverse habitats such as soil, fresh and marine waters and plants and cluster-II comprising Anaplasma sp. and Erlichia, and Wolbachia endosymbionts of insects and nematodes, in distinct groups.

CONCLUSIONS: HtrA-type serine protease from Wolbachia of W. bancrofti is highly conserved among filarial parasites. It will be of interest to know whether filarial Wolbachia HtrA type of serine protease might influence apoptosis and lymphatic epithelium, thereby playing a role in the filarial pathogenesis. Such information will be useful for identifying targets for the development of newer drugs for filariasis treatment, especially for preventing lymphatic pathology.

Keywords: HtrA serine protease; Wolbachia; Wuchereria bancrofti; lymphatic filariasis

References

  1. J Clin Invest. 1999 Jan;103(1):11-8 - PubMed
  2. Biochem Biophys Res Commun. 1991 Apr 30;176(2):730-6 - PubMed
  3. Parasitology. 2001 Jan;122 Pt 1:93-103 - PubMed
  4. Parasitology. 2006 Nov;133(Pt 5):589-601 - PubMed
  5. J Bacteriol. 1989 May;171(5):2689-96 - PubMed
  6. Int J Parasitol. 2002 Nov;32(12):1457-68 - PubMed
  7. J Parasitol. 2002 Jun;88(3):605-11 - PubMed
  8. Indian J Med Res. 2005 Sep;122(3):199-204 - PubMed
  9. Microbes Infect. 2004 Feb;6(2):141-9 - PubMed
  10. Mol Biochem Parasitol. 1995 Nov;74(2):223-7 - PubMed
  11. Parasitol Today. 1999 Nov;15(11):437-42 - PubMed
  12. PLoS Biol. 2004 Mar;2(3):E69 - PubMed
  13. Arch Med Res. 2002 Jul-Aug;33(4):422-4 - PubMed
  14. Cell Death Differ. 2002 Apr;9(4):394-404 - PubMed
  15. Lancet. 2000 Apr 8;355(9211):1242-3 - PubMed
  16. Parasitol Today. 1999 Nov;15(11):428-9 - PubMed
  17. Proc Biol Sci. 2000 Jun 7;267(1448):1063-9 - PubMed
  18. J Infect Dis. 2002 Mar 15;185(6):805-11 - PubMed
  19. Curr Mol Med. 2002 May;2(3):299-302 - PubMed
  20. Microbes Infect. 2000 Aug;2(10):1147-57 - PubMed
  21. Curr Opin Investig Drugs. 2002 Apr;3(4):533-7 - PubMed
  22. Acta Trop. 2006 Aug;99(1):1-5 - PubMed
  23. Mol Biol Evol. 2002 Mar;19(3):341-6 - PubMed
  24. Vet Parasitol. 2001 Jul 12;98(1-3):215-38 - PubMed
  25. Int J Parasitol. 2009 Jul 15;39(9):1045-54 - PubMed
  26. Annu Rev Entomol. 1997;42:587-609 - PubMed
  27. Indian J Med Res. 1984 Apr;79:497-501 - PubMed
  28. Lancet. 2001 Dec 1;358(9296):1873-5 - PubMed
  29. Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2699-702 - PubMed
  30. Vet Parasitol. 2002 Sep 10;108(2):153-61 - PubMed
  31. Lancet. 2005 Jun 18-24;365(9477):2116-21 - PubMed
  32. Exp Parasitol. 2000 Aug;95(4):265-70 - PubMed
  33. Parasitol Today. 2000 May;16(5):179-80 - PubMed
  34. Trop Med Int Health. 2001 Nov;6(11):915-21 - PubMed
  35. Nucleic Acids Res. 1988 Nov 11;16(21):10053-67 - PubMed
  36. J Bacteriol. 1989 Mar;171(3):1574-84 - PubMed
  37. Mol Biol Evol. 2007 Aug;24(8):1596-9 - PubMed
  38. Proc Biol Sci. 1998 Dec 22;265(1413):2407-13 - PubMed
  39. Ann Trop Med Parasitol. 2000 Dec;94(8):801-16 - PubMed
  40. Curr Opin Infect Dis. 2001 Dec;14(6):727-31 - PubMed
  41. Trop Med Int Health. 2000 Apr;5(4):275-9 - PubMed
  42. Cell. 1998 Jun 12;93(6):961-72 - PubMed
  43. Mem Inst Oswaldo Cruz. 2003 Dec;98(8):1017-9 - PubMed
  44. Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12706-11 - PubMed
  45. Trans R Soc Trop Med Hyg. 2000 Nov-Dec;94(6):596-8 - PubMed
  46. Appl Environ Microbiol. 2005 Jun;71(6):3205-12 - PubMed
  47. Int J Parasitol. 1999 Feb;29(2):357-64 - PubMed
  48. Mol Biochem Parasitol. 1998 Oct 30;96(1-2):69-81 - PubMed
  49. Trans R Soc Trop Med Hyg. 1975;69(5-6):509-14 - PubMed

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