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Sato S, Morita N, Kitamoto D, et al. Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol. AMB Express. 2013;3(1):20doi: 10.1186/2191-0855-3-20.
Sato, S., Morita, N., Kitamoto, D., Yakushi, T., Matsushita, K., & Habe, H. (2013). Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol. AMB Express, 3(1), 20. https://doi.org/10.1186/2191-0855-3-20
Sato, Shun, et al. "Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol." AMB Express vol. 3,1 (2013): 20. doi: https://doi.org/10.1186/2191-0855-3-20
Sato S, Morita N, Kitamoto D, Yakushi T, Matsushita K, Habe H. Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol. AMB Express. 2013 Apr 02;3(1):20. doi: 10.1186/2191-0855-3-20. PMID: 23547945; PMCID: PMC3627625.
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AMB Express. 2013 Apr 02;3(1):20. doi: 10.1186/2191-0855-3-20.

Change in product selectivity during the production of glyceric acid from glycerol by Gluconobacter strains in the presence of methanol.

AMB Express

Shun Sato, Naoki Morita, Dai Kitamoto, Toshiharu Yakushi, Kazunobu Matsushita, Hiroshi Habe

Affiliations

  1. Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan. [email protected].

PMID: 23547945 PMCID: PMC3627625 DOI: 10.1186/2191-0855-3-20

Abstract

To enhance the value-added use of methanol-containing raw glycerol derived from biodiesel fuel production, the effect of methanol supplementation on glyceric acid (GA) production by Gluconobacter spp. was investigated. We first conducted fed-batch fermentation with Gluconobacter frateurii NBRC103465 using raw glycerol as a feeding solution. GA productivity decreased with increasing dihydroxyacetone (DHA) formation when the raw glycerol contained methanol. The results of this experiment and comparative experiments using a synthetic solution modeled after the raw glycerol indicate that the presence of methanol caused a change in the concentrations of GA and DHA, two glycerol derivatives produced during fermentation. Other Gluconobacter spp. also decreased GA production in the presence of 1% (v/v) methanol. In addition, purified membrane-bound alcohol dehydrogenase (mADH) from Gluconobacter oxydans, which is a key enzyme in GA production, showed a decrease in dehydrogenase activity toward glycerol as the methanol concentration increased. These results strongly suggest that the observed decrease in GA production by Gluconobacter spp. resulted from the methanol-induced inhibition of mADH-mediated glycerol oxidation.

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