Display options
Cite
Wang X, Wang B, Zhang P, et al. The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens. Springerplus. 2013;2(1):138doi: 10.1186/2193-1801-2-138.
Wang, X., Wang, B., Zhang, P., Cheng, H., & Sun, S. (2013). The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens. SpringerPlus, 2(1), 138. https://doi.org/10.1186/2193-1801-2-138
Wang, Xiuzhen, et al. "The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens." SpringerPlus vol. 2,1 (2013): 138. doi: https://doi.org/10.1186/2193-1801-2-138
Wang X, Wang B, Zhang P, Cheng H, Sun S. The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens. Springerplus. 2013 Mar 29;2(1):138. doi: 10.1186/2193-1801-2-138. Print 2013 Dec. PMID: 23607085; PMCID: PMC3627852.
Copy Download .nbib Format: NLM
Share it on

Springerplus. 2013 Mar 29;2(1):138. doi: 10.1186/2193-1801-2-138. Print 2013 Dec.

The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens.

SpringerPlus

Xiuzhen Wang, Bo Wang, Peipei Zhang, Hegang Cheng, Shuhong Sun

Affiliations

  1. College of Veterinary Medicine, Shandong Agricultural University, Taian, Shandong PR China.

PMID: 23607085 PMCID: PMC3627852 DOI: 10.1186/2193-1801-2-138

Abstract

Avian leukosis (AL) is one of the most harmful diseases to the poultry industry in China. The detection of the avian leukosis virus (ALV) p27 antigen plays a decisive role in the elimination of avian leukosis. To explore the influence of passaging cells on the detection rate of the ALV p27 antigen, 21 aseptic anticoagulated blood samples were collected from 21 chickens for which the cloacal swabs were positive for the p27 antigen to inoculate two sets of cell culture plates containing DF1 cells. The cells were cultured for 4 d, one set was passaged, and the other set was not. After the DF1 cells had been cultured for 9 d, the ALV p27 antigen in the supernatants of the two sets was detected by ELISA. The results showed that the p27 antigen-positive rate for the passaged cells was 71.43% (15/21), higher than that of the cells that were directly cultured, which was 42.86%. There was a strong correlation, as high as 0.928, with respect to the S/P value of the p27 antigen in the supernatant between the two sets. In conclusion, there was a strong correlation between the results for the passaged and unpassaged cells, and the passage of cells greatly improved the detection of the p27 antigen.

Keywords: Avian leukosis virus; Cells passage; Detection rate of p27 antigen

References

  1. Wei Sheng Wu Xue Bao. 2005 Aug;45(4):584-7 - PubMed
  2. Avian Pathol. 2007 Jun;36(3):221-6 - PubMed
  3. Avian Pathol. 1976;5(3):221-6 - PubMed
  4. Acta Vet Hung. 1997;45(3):251-66 - PubMed
  5. Bing Du Xue Bao. 2010 Jan;26(1):53-7 - PubMed
  6. Avian Pathol. 1984 Apr;13(2):321-32 - PubMed
  7. Avian Dis. 1984 Apr-Jun;28(2):358-73 - PubMed
  8. Avian Dis. 2012 Mar;56(1):35-43 - PubMed

Publication Types