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Watanabe S, Isoda N, Ohtake T, et al. Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A. Hepatol Res. 2013;44(3):270-279doi: 10.1111/hepr.12124.
Watanabe, S., Isoda, N., Ohtake, T., Hirosawa, T., Morimoto, N., Aoki, K., Ohnishi, H., Takahashi, M., Sugano, K., & Okamoto, H. (2014). Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A. Hepatology research : the official journal of the Japan Society of Hepatology, 44(3), 270-279. https://doi.org/10.1111/hepr.12124
Watanabe, Shunji, et al. "Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A." Hepatology research : the official journal of the Japan Society of Hepatology vol. 44,3 (2014): 270-279. doi: https://doi.org/10.1111/hepr.12124
Watanabe S, Isoda N, Ohtake T, Hirosawa T, Morimoto N, Aoki K, Ohnishi H, Takahashi M, Sugano K, Okamoto H. Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A. Hepatol Res. 2014 Mar;44(3):270-279. doi: 10.1111/hepr.12124. Epub 2013 Apr 29. PMID: 23607583.
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Hepatol Res. 2014 Mar;44(3):270-279. doi: 10.1111/hepr.12124. Epub 2013 Apr 29.

Full genome analysis of Philippine indigenous subgenotype IA hepatitis A virus strains from Japanese patients with imported acute hepatitis A.

Hepatology research : the official journal of the Japan Society of Hepatology

Shunji Watanabe, Norio Isoda, Toshiya Ohtake, Takuya Hirosawa, Naoki Morimoto, Kei Aoki, Hiroshi Ohnishi, Masaharu Takahashi, Kentaro Sugano, Hiroaki Okamoto

Affiliations

  1. Division of Gastroenterology and Hepatology, Department of Internal Medicine, Jichi Medical University School of Medicine, Shimotsuke, Japan.

PMID: 23607583 DOI: 10.1111/hepr.12124

Abstract

AIM: Hepatitis A virus (HAV) is the most common cause of infectious hepatitis worldwide. Although hepatitis A cases imported from South-East Asian countries, including the Philippines, have been reported in Japan, the molecular epidemiological data have been limited for these HAV-endemic countries.

METHODS: The full-length genomic sequences of HAV isolates were determined and subjected to the phylogenetic analyses.

RESULTS: The HAV isolates (HA12-0796 and HA12-0938) obtained from two Japanese patients who developed acute hepatitis A in July 2012, 1 month after traveling to the Philippines, where they consumed undercooked shellfish, differed by only one nucleotide (nt) over the entire genome. These HAV isolates of genotype IA were 99.1-99.5% identical within 228-237 nt to those recovered from river water in the Philippines, suggesting that the HA12-0796 and HA12-0938 isolates represent HAV circulating in the Philippines. HAV isolates belonging to one of the two IA sublineages (IA-2) which were implicated in some of the mini-epidemics in 2010 in Japan are hypothesized to be connected with the Philippines. In support of this speculation, the present IA isolates (HA12-0796 and HA12-0938) shared 98.8% identity over the entire genome with one IA-2 isolate (HAJIH-Fukuo10) recovered from a Japanese female who developed a domestic HAV infection during the mini-epidemics. In the phylogenetic tree constructed based on the entire genome, these three isolates (HA12-0796, HA12-0938 and HAJIH-Fukuo10) segregated into a cluster with a bootstrap value of 100%.

CONCLUSION: These results indicate that HAV isolates belonging to the IA-2 lineage might have been imported from the Philippines.

© 2013 The Japan Society of Hepatology.

Keywords: Philippines; complete genome; hepatitis A; hepatitis A virus; phylogenetic analysis

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