Iran J Basic Med Sci. 2011 Nov;14(6):560-7.

Effects of Low-dose Morphine on Nitric Oxide Concentration and Angiogenesis in Two-kidney One Clip Hypertensive Rats.

Iranian journal of basic medical sciences

Aliasghar Pourshanazari, Mohammad Allahtavakoli, Ghgholamhossein Hassanshahi

PMID: 23493527 PMCID: PMC3586858

Abstract

OBJECTIVES: We investigated the effects of low-dose morphine on nitric oxide (NO) and angiogenesis in two-kidney one clip hypertensive (2K1C) rats.

MATERIALS AND METHODS: MALE RATS WERE DIVIDED INTO TWO GROUPS: sham-clip operated and 2K1C. Each group subdivided into saline and morphine (3 mg/kg i.p. 8 weeks) groups. Blood pressure, heart rate, plasma renin activity (PRA), NO concentration and murine matrigel angiogenesis were evaluated.

RESULTS: Morphine had no effects on blood pressures and HR in sham normotensive rats but attenuated diastolic blood pressure (DBP) (P< 0.01) and mean arterial pressure (MAP) (P< 0.01) in 2K1C compared with saline. PRA level was significantly higher in 2K1C compared with sham groups (P< 0.01) but morphine decreased it in 2K1C compared with saline (P< 0.01). After clipping, NO in 2K1C hypertensive rats was decreased (P< 0.01) and morphine increased it compared with saline (P< 0.01). Morphine promoted angiogenesis in both sham (P< 0.01) and 2K1C (P< 0.0001) groups.

CONCLUSION: Low-dose morphine stimulated angiogenesis in two-kidney one clip hypertensive rats probably via NO pathways.

Keywords: Angiogenesis; Blood pressure; Morphine; Nitric oxide; Renin activity

References

1. Anesthesiology. 2007 Mar;106(3):532-7 - PubMed
2. J Pharmacol Exp Ther. 1990 May;253(2):646-54 - PubMed
3. Hypertension. 1991 May;17(5):707-19 - PubMed
4. J Biol Chem. 1998 May 15;273(20):12402-6 - PubMed
5. Pharmacology. 2006;77(1):17-24 - PubMed
6. Med Oncol. 2011 Dec;28(4):1264-72 - PubMed
7. Curr Neurovasc Res. 2006 Aug;3(3):171-80 - PubMed
8. J Mol Cell Cardiol. 1997 Jan;29(1):55-65 - PubMed
9. J Leukoc Biol. 2004 Jun;75(6):1131-8 - PubMed
10. J Res Med Sci. 2011 Mar;16(3):269-75 - PubMed
11. Angiogenesis. 2004;7(3):193-201 - PubMed
12. Circ Res. 2004 Apr 16;94(7):960-6 - PubMed
13. J Pharmacol Exp Ther. 1995 Jan;272(1):1-7 - PubMed
14. J Neuroimmunol. 2004 Jan;146(1-2):13-21 - PubMed
15. J Clin Hypertens (Greenwich). 2008 Apr;10(4):304-10 - PubMed
16. Exp Eye Res. 2009 Nov;89(5):660-4 - PubMed
17. Hypertension. 1987 Jun;9(6 Pt 2):III193-7 - PubMed
18. Clin Chem. 2003 Jan;49(1):32-40 - PubMed
19. Hypertens Res. 2008 May;31(5):873-9 - PubMed
20. Inflammation. 1994 Jun;18(3):293-9 - PubMed
21. Int J Immunopharmacol. 2000 Aug;22(8):603-14 - PubMed
22. Exp Eye Res. 2007 Jan;84(1):185-90 - PubMed
23. Anesth Analg. 2008 Aug;107(2):686-92 - PubMed
24. Am J Hypertens. 2003 Jan;16(1):11-20 - PubMed
25. Nitric Oxide. 2001 Feb;5(1):62-71 - PubMed
26. Acta Physiol (Oxf). 2009 Jun;196(2):193-222 - PubMed
27. J Cereb Blood Flow Metab. 2001 Aug;21(8):937-44 - PubMed
28. J Biol Chem. 1995 Dec 22;270(51):30290-3 - PubMed
29. J Cardiovasc Pharmacol. 2010 Apr;55(4):300-7 - PubMed
30. Regul Pept. 2001 Jun 15;99(2-3):87-92 - PubMed
31. Surgery. 2003 Aug;134(2):336-44 - PubMed
32. J Anesth. 2009;23(4):554-68 - PubMed
33. Am J Physiol Heart Circ Physiol. 2003 Jul;285(1):H81-9 - PubMed
34. Acta Physiol Scand. 2002 Jul;175(3):245-51 - PubMed
35. J Hum Hypertens. 2010 Nov;24(11):713-20 - PubMed
36. Pathophysiology. 2011 Jun;18(3):201-6 - PubMed
37. Cancer Res. 2002 Aug 1;62(15):4491-8 - PubMed
38. J Hypertens. 2005 Nov;23(11):2013-8 - PubMed
39. J Biol Chem. 1998 Sep 4;273(36):23534-41 - PubMed
40. Anesthesiology. 2009 Jan;110(1):166-81 - PubMed
41. Gen Pharmacol. 1991;22(6):1077-9 - PubMed
42. Nat Med. 2009 Jun;15(6):657-64 - PubMed
43. Heart Fail Rev. 2002 Oct;7(4):347-58 - PubMed

Publication Types

• Journal Article