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Keeney JB, Reed R. A Genetics Laboratory Module Involving Selection and Identification of Lysine Synthesis Mutants in the Yeast Saccharomyces cerevisiae. Microbiol Educ. 2000;1:26-30doi: 10.1128/me.1.1.26-30.2000.
Keeney, J. B., & Reed, R. (2000). A Genetics Laboratory Module Involving Selection and Identification of Lysine Synthesis Mutants in the Yeast Saccharomyces cerevisiae. Microbiology education, 126-30. https://doi.org/10.1128/me.1.1.26-30.2000
Keeney, J B, and Reed, R. "A Genetics Laboratory Module Involving Selection and Identification of Lysine Synthesis Mutants in the Yeast Saccharomyces cerevisiae." Microbiology education vol. 1 (2000): 26-30. doi: https://doi.org/10.1128/me.1.1.26-30.2000
Keeney JB, Reed R. A Genetics Laboratory Module Involving Selection and Identification of Lysine Synthesis Mutants in the Yeast Saccharomyces cerevisiae. Microbiol Educ. 2000 May;1:26-30. doi: 10.1128/me.1.1.26-30.2000. PMID: 23653536; PMCID: PMC3633095.
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Microbiol Educ. 2000 May;1:26-30. doi: 10.1128/me.1.1.26-30.2000.

A Genetics Laboratory Module Involving Selection and Identification of Lysine Synthesis Mutants in the Yeast Saccharomyces cerevisiae.

Microbiology education

J B Keeney, R Reed

Affiliations

  1. Department of Biology and.

PMID: 23653536 PMCID: PMC3633095 DOI: 10.1128/me.1.1.26-30.2000

Abstract

We have developed a laboratory exercise, currently being used with college sophomores, which uses the yeast Saccharomyces cerevisiae to convey the concepts of amino acid biosynthesis, mutation, and gene complementation. In brief, selective medium is used to isolate yeast cells carrying a mutation in the lysine biosynthesis pathway. A spontaneous mutation in any one of three separate genetic loci will allow for growth on selective media; however, the frequency of mutations isolated from each locus differs. Following isolation of a mutated strain, students use complementation analysis to identify which gene contains the mutation. Since the yeast genome has been mapped and sequenced, students with access to the Internet can then research and develop hypotheses to explain the differences in frequencies of mutant genes obtained.

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