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Bloem K, García-Vallejo JJ, Vuist IM, et al. Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells. Front Immunol. 2013;4:103doi: 10.3389/fimmu.2013.00103.
Bloem, K., García-Vallejo, J. J., Vuist, I. M., Cobb, B. A., van Vliet, S. J., & van Kooyk, Y. (2013). Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells. Frontiers in immunology, 4103. https://doi.org/10.3389/fimmu.2013.00103
Bloem, Karien, et al. "Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells." Frontiers in immunology vol. 4 (2013): 103. doi: https://doi.org/10.3389/fimmu.2013.00103
Bloem K, García-Vallejo JJ, Vuist IM, Cobb BA, van Vliet SJ, van Kooyk Y. Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells. Front Immunol. 2013 May 06;4:103. doi: 10.3389/fimmu.2013.00103. eCollection 2013. PMID: 23653626; PMCID: PMC3644800.
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Front Immunol. 2013 May 06;4:103. doi: 10.3389/fimmu.2013.00103. eCollection 2013.

Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells.

Frontiers in immunology

Karien Bloem, Juan J García-Vallejo, Ilona M Vuist, Brian A Cobb, Sandra J van Vliet, Yvette van Kooyk

Affiliations

  1. Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, Netherlands ; Centre for Specialized Nutrition, Danone Research Wageningen, Netherlands.

PMID: 23653626 PMCID: PMC3644800 DOI: 10.3389/fimmu.2013.00103

Abstract

The zwitterionic capsular polysaccharide A (PSA) of Bacteroides fragilis is the first carbohydrate antigen described to be presented in major histocompatibility complex (MHC) class II for the induction of CD4(+) T cell responses. However, the identity of the receptor mediating binding and internalization of PSA in antigen presenting cells remains elusive. C-type lectins are glycan-binding receptors known for their capacity to target ligands for antigen presentation to T cells. Here, we investigated whether C-type lectins were involved in the internalization of PSA and identified dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) as the main receptor for PSA on human dendritic cells (DC). The induction of PSA-specific T cell proliferation appeared to be completely dependent on DC-SIGN. These data reveal a crucial role for DC-SIGN in the endocytosis and routing of PSA in human DC for the efficient stimulation of PSA-specific CD4(+) T cells.

Keywords: C-type lectin; T cell proliferation; dendritic cell; pathogen; polysaccharide

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