Display options
Cite
Zou L, Yan S, Guan X, et al. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. J Diabetes Res. 2013;2013:721493doi: 10.1155/2013/721493.
Zou, L., Yan, S., Guan, X., Pan, Y., & Qu, X. (2013). Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. Journal of diabetes research, 2013721493. https://doi.org/10.1155/2013/721493
Zou, Li, et al. "Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus." Journal of diabetes research vol. 2013 (2013): 721493. doi: https://doi.org/10.1155/2013/721493
Zou L, Yan S, Guan X, Pan Y, Qu X. Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus. J Diabetes Res. 2013;2013:721493. doi: 10.1155/2013/721493. Epub 2013 Mar 16. PMID: 23671888; PMCID: PMC3647574.
Copy Download .nbib Format: NLM
Share it on

J Diabetes Res. 2013;2013:721493. doi: 10.1155/2013/721493. Epub 2013 Mar 16.

Hypermethylation of the PRKCZ Gene in Type 2 Diabetes Mellitus.

Journal of diabetes research

Li Zou, Shirong Yan, Xueping Guan, Yunjun Pan, Xin Qu

Affiliations

  1. Clinical Laboratory of Renmin Hospital, The Third Affiliated Hospital of Hubei University of Medicine, 39 Chaoyang Road, Shiyan, Hubei Province 442000, China.

PMID: 23671888 PMCID: PMC3647574 DOI: 10.1155/2013/721493

Abstract

Objectives. To study the correlation between the methylation of protein kinase C epsilon zeta (PRKCZ) gene promoters and type 2 diabetes mellitus (T2DM). Methods. The case-control method was implemented in 272 unrelated to one another cases in Shiyan People's Hospital. Of those, 152 were diagnosed as T2DM cases, and the other 120 cases were healthy individuals visiting the hospital for a physical examination. The subjects were first divided into two groups: the T2DM group and the normal control (NC) group. Next, methylated DNA immunoprecipitation chip (MeDIP-chip) was used for detection. Bisulfite sequencing PCR (BSP) and gene sequencing were then performed to detect and analyze the correlation between PRKCZ gene promoter methylation and T2DM. Finally, Western blotting was applied to determine the serum level of PRKCZ. The data were then analyzed with the statistics analyzing software SPSS 17.0. Results. In contrast with cases in NC, T2DM patients showed a high level of methylation, with 7 of 9 CpG sites were shown to be methylated, whereas, in the control group, only one CpG site was found to be methylated. The methylated CpG sites for the two groups showed marked differences (P < 0.01). Additionally, the level of PRKCZ was decreased in T2DM subjects, and the difference between the two groups was statistically significant (P < 0.05). Discussion. This study suggests that the PRKCZ gene is the hypermethylated gene of T2DM and the hypermethylation PRKCZ gene may be involved in the pathogenesis of T2DM.

References

  1. Hum Mol Genet. 2000 Mar 1;9(4):589-96 - PubMed
  2. J Nutr. 2002 Aug;132(8 Suppl):2440S-2443S - PubMed
  3. Nucleic Acids Res. 2001 Nov 15;29(22):4598-606 - PubMed
  4. BMC Biol. 2009 Jul 10;7:38 - PubMed
  5. PLoS One. 2009 Sep 09;4(9):e6953 - PubMed
  6. J Biol Chem. 2007 Jun 22;282(25):18018-18027 - PubMed
  7. Nat Rev Genet. 2002 Jun;3(6):415-28 - PubMed
  8. J Clin Invest. 2000 Feb;105(4):401-7 - PubMed
  9. Diabetes Care. 2004 May;27(5):1047-53 - PubMed
  10. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;34(9):837-45 - PubMed
  11. J Nutr. 2002 Aug;132(8 Suppl):2373S-2376S - PubMed
  12. World J Gastroenterol. 2003 Sep;9(9):2078-82 - PubMed
  13. Diabetes. 2002 May;51(5):1409-18 - PubMed
  14. Science. 2001 Aug 10;293(5532):1064-7 - PubMed
  15. Diabetologia. 2008 Apr;51(4):615-22 - PubMed
  16. Diabetes Metab Res Rev. 2008 Sep;24(6):480-5 - PubMed

Publication Types