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Fassan M, Volinia S, Palatini J, et al. MicroRNA Expression Profiling in the Histological Subtypes of Barrett's Metaplasia. Clin Transl Gastroenterol. 2013;4:e34doi: 10.1038/ctg.2013.5.
Fassan, M., Volinia, S., Palatini, J., Pizzi, M., Fernandez-Cymering, C., Balistreri, M., Realdon, S., Battaglia, G., Souza, R., Odze, R. D., Zaninotto, G., Croce, C. M., & Rugge Md Facg, M. (2013). MicroRNA Expression Profiling in the Histological Subtypes of Barrett's Metaplasia. Clinical and translational gastroenterology, 4e34. https://doi.org/10.1038/ctg.2013.5
Fassan, Matteo, et al. "MicroRNA Expression Profiling in the Histological Subtypes of Barrett's Metaplasia." Clinical and translational gastroenterology vol. 4 (2013): e34. doi: https://doi.org/10.1038/ctg.2013.5
Fassan M, Volinia S, Palatini J, Pizzi M, Fernandez-Cymering C, Balistreri M, Realdon S, Battaglia G, Souza R, Odze RD, Zaninotto G, Croce CM, Rugge Md Facg M. MicroRNA Expression Profiling in the Histological Subtypes of Barrett's Metaplasia. Clin Transl Gastroenterol. 2013 May 16;4:e34. doi: 10.1038/ctg.2013.5. PMID: 23677165; PMCID: PMC3671360.
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Clin Transl Gastroenterol. 2013 May 16;4:e34. doi: 10.1038/ctg.2013.5.

MicroRNA Expression Profiling in the Histological Subtypes of Barrett's Metaplasia.

Clinical and translational gastroenterology

Matteo Fassan, Stefano Volinia, Jeff Palatini, Marco Pizzi, Cecilia Fernandez-Cymering, Mariangela Balistreri, Stefano Realdon, Giorgio Battaglia, Rhonda Souza, Robert D Odze, Giovanni Zaninotto, Carlo M Croce, Massimo Rugge Md Facg

Affiliations

  1. 1] Department of Medicine (DIMED), Surgical Pathology and Cytopathology Unit, University of Padua, Padua, Italy [2] Department of Surgical, Oncological and Gastroenterological Sciences (DiSCOG), General Oncology Unit, University of Padua, Padua, Italy.

PMID: 23677165 PMCID: PMC3671360 DOI: 10.1038/ctg.2013.5

Abstract

OBJECTIVES: The histological definition of Barrett's esophagus (BE) is debated, particularly regarding the phenotype of its metaplastic columnar epithelium. Histologically proven intestinal metaplasia (IM) was the sine qua non condition for a diagnosis of BE but, more recently, non-intestinalized (i.e., cardiac gastric-type; GM) columnar metaplasia has been re-included in the spectrum of Barrett's histology. MicroRNAs modulate cell commitment, and are also reportedly dysregulated in Barrett's carcinogenesis. This study investigates miRNA expression in the histological spectrum of esophageal columnar metaplastic changes, specifically addressing the biological profile of GM vs. IM.

METHODS: A study was performed to discover microRNA microarray in 30 matching mucosa samples obtained from 10 consecutive BE patients; for each patient, biopsy tissue samples were obtained from squamous, GM and intestinalized epithelium. Microarray findings were further validated by qRT-PCR analysis in another bioptic series of 75 mucosa samples.

RESULTS: MicroRNA profiling consistently disclosed metaplasia-specific microRNA signatures. Six microRNAs were significantly dysregulated across the histological phenotypes considered; five of them (two overexpressed (hsa-miR-192; -miR-215) and three under-expressed (hsa-miR-18a*; -miR-203, and -miR-205)) were progressively dysregulated in the phenotypic sequence from squamous to gastric-type, to intestinal-type mucosa samples.

CONCLUSIONS: A consistent microRNA expression signature underlies both gastric- and intestinal-type esophageal metaplasia. The pattern of microRNA dysregulation suggests that GM may further progress to IM. The clinico-pathological implications of these molecular profiles prompt further study on the "personalized" cancer risk associated with each of these metaplastic transformations.

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