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Oakes ND, Kjellstedt A, Thalén P, et al. Roles of Fatty Acid oversupply and impaired oxidation in lipid accumulation in tissues of obese rats. J Lipids. 2013;2013:420754doi: 10.1155/2013/420754.
Oakes, N. D., Kjellstedt, A., Thalén, P., Ljung, B., & Turner, N. (2013). Roles of Fatty Acid oversupply and impaired oxidation in lipid accumulation in tissues of obese rats. Journal of lipids, 2013420754. https://doi.org/10.1155/2013/420754
Oakes, Nicholas D, et al. "Roles of Fatty Acid oversupply and impaired oxidation in lipid accumulation in tissues of obese rats." Journal of lipids vol. 2013 (2013): 420754. doi: https://doi.org/10.1155/2013/420754
Oakes ND, Kjellstedt A, Thalén P, Ljung B, Turner N. Roles of Fatty Acid oversupply and impaired oxidation in lipid accumulation in tissues of obese rats. J Lipids. 2013;2013:420754. doi: 10.1155/2013/420754. Epub 2013 May 13. PMID: 23762564; PMCID: PMC3666279.
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J Lipids. 2013;2013:420754. doi: 10.1155/2013/420754. Epub 2013 May 13.

Roles of Fatty Acid oversupply and impaired oxidation in lipid accumulation in tissues of obese rats.

Journal of lipids

Nicholas D Oakes, Ann Kjellstedt, Pia Thalén, Bengt Ljung, Nigel Turner

Affiliations

  1. AstraZeneca R&D Mölndal, 431 83 Mölndal, Sweden.

PMID: 23762564 PMCID: PMC3666279 DOI: 10.1155/2013/420754

Abstract

To test the roles of lipid oversupply versus oxidation in causing tissue lipid accumulation associated with insulin resistance/obesity, we studied in vivo fatty acid (FA) metabolism in obese (Obese) and lean (Lean) Zucker rats. Indices of local FA utilization and storage were calculated using the partially metabolizable [9,10-(3)H]-(R)-2-bromopalmitate ((3)H-R-BrP) and [U-(14)C]-palmitate ((14)C-P) FA tracers, respectively. Whole-body FA appearance (R a ) was estimated from plasma (14)C-P kinetics. Whole-body FA oxidation rate (R ox) was assessed using (3)H2O production from (3)H-palmitate infusion, and tissue FA oxidative capacity was evaluated ex vivo. In the basal fasting state Obese had markedly elevated FA levels and R a , associated with elevated FA utilization and storage in most tissues. Estimated rates of muscle FA oxidation were not lower in obese rats and were similarly enhanced by contraction in both lean and obese groups. At comparable levels of FA availability, achieved by nicotinic acid, R ox was lower in Obese than Lean. In Obese rats, FA oxidative capacity was 35% higher than that in Lean in skeletal muscle, 67% lower in brown fat and comparable in other organs. In conclusion, lipid accumulation in non-adipose tissues of obese Zucker rats appears to result largely from systemic FA oversupply.

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