J Sep Sci. 2013 Sep;36(17):2901-14. doi: 10.1002/jssc.201300458. Epub 2013 Jul 30.

GC-MS study of compounds isolated from Coffea arabica flowers by different extraction techniques.

Journal of separation science

Elena E Stashenko, Jairo René Martínez, Silvia Cárdenas-Vargas, Rogerio Saavedra-Barrera, Diego Camilo Durán

PMID: 23801537 DOI: 10.1002/jssc.201300458

Abstract

Headspace (HS), extractive, and distillative methods were employed to isolate volatile and semivolatile compounds from fresh Coffea arabica flowers. Static HS solid-phase microextraction (HS-SPME), microwave-assisted HS-SPME (MW-HS-SPME) with simultaneous hydrodistillation, and extraction with hexane or supercritical CO2 -isolated mixtures in which around 150 different chemical substances were identified or tentatively identified by GC-MS analysis. n-Pentadecane (20-37% relative peak area, RPA) was the most abundant compound in the HS fractions from fresh flowers, followed by 8-heptadecene (8-20% RPA) and geraniol (6-14% RPA). Hydrocarbons (mostly C13 -C30 paraffins) were the predominant compound class in all the sorptive extractions (HS-SPME, MW-HS-SPME, distillate), followed by terpenoids or oxygenated compounds (which varied with the isolation technique). Caffeine, a distinctive component of coffee fruits and beans, was also found in relatively high amounts in the supercritical CO2 extract of C. arabica flowers.

© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: Coffea arabica; Coffee flower compounds; Microwave-assisted extraction; Solid-phase microextraction; Supercritical fluid extraction

MeSH terms

• Coffea / chemistry
• Flowers / chemistry
• Gas Chromatography-Mass Spectrometry / methods
• Solid Phase Microextraction

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't