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Karra VK, Pilli NR, Inamadugu JK, et al. Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study. Pharm Methods. 2012;3(1):18-25doi: 10.4103/2229-4708.97711.
Karra, V. K., Pilli, N. R., Inamadugu, J. K., & Rao, J. V. (2012). Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study. Pharmaceutical methods, 3(1), 18-25. https://doi.org/10.4103/2229-4708.97711
Karra, Vijaya Kumari, et al. "Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study." Pharmaceutical methods vol. 3,1 (2012): 18-25. doi: https://doi.org/10.4103/2229-4708.97711
Karra VK, Pilli NR, Inamadugu JK, Rao JV. Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study. Pharm Methods. 2012 Jan;3(1):18-25. doi: 10.4103/2229-4708.97711. PMID: 23781473; PMCID: PMC3658069.
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Pharm Methods. 2012 Jan;3(1):18-25. doi: 10.4103/2229-4708.97711.

Simultaneous determination of losartan, losartan acid and amlodipine in human plasma by LC-MS/MS and its application to a human pharmacokinetic study.

Pharmaceutical methods

Vijaya Kumari Karra, Nageswara Rao Pilli, Jaswanth Kumar Inamadugu, J V L N Seshagiri Rao

Affiliations

  1. University College of Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, Andhra Pradesh, India.

PMID: 23781473 PMCID: PMC3658069 DOI: 10.4103/2229-4708.97711

Abstract

INTRODUCTION: A simple, rapid and sensitive liquid chromatography-tandem mass spectrometric assay method has been developed and fully validated for simultaneous quantification of losartan and its active metabolite, losartan carboxylic acid, and amlodipine in human plasma. Irbesartan was used as an internal standard.

MATERIALS AND METHODS: The analytes were extracted from human plasma samples by solid-phase extraction technique using Oasis HLB cartridges, (Waters Corporation, Mumbai, India). The reconstituted samples were chromatographed on a C18 column by using an 85:15, v/v mixture of methanol and 0.1% v/v formic acid as the mobile phase at a flow rate of 1.0 mL/min. A detailed validation of the method was performed as per the FDA guidelines.

RESULTS: The calibration curves obtained were linear (r ≥ 0.99) over the concentration range of 0.5-1000 ng/mL for losartan and for its active metabolite losartan acid and 0.05-10.1 ng/mL for amlodipine. The results of the intra- and inter-day precision and accuracy studies were well within the acceptable limits.

CONCLUSIONS: A run time of 2.5 min for each sample made it possible to analyze more than 300 plasma samples per day. The proposed method was found to be applicable to clinical studies.

Keywords: Amlodipine; liquid chromatography-tandem mass spectrometric method; losartan; losartan acid; pharmacokinetics

References

  1. Clin Ther. 1996 Jul-Aug;18(4):608-25 - PubMed
  2. Drugs. 1991 Mar;41(3):478-505 - PubMed
  3. Eur J Pharm Sci. 2008 Jan;33(1):91-9 - PubMed
  4. Arch Pharm Res. 2009 Mar;32(3):353-8 - PubMed
  5. Biomed Chromatogr. 2007 Feb;21(2):169-75 - PubMed
  6. Hypertension. 2005 Aug;46(2):386-92 - PubMed
  7. J Pharm Biomed Anal. 2009 Apr 5;49(3):862-7 - PubMed
  8. Biomed Chromatogr. 2006 Sep;20(9):833-42 - PubMed
  9. Am Heart J. 1989 Nov;118(5 Pt 2):1100-3 - PubMed
  10. J Pharm Biomed Anal. 2003 Sep 15;33(1):73-84 - PubMed
  11. Circulation. 2001 Feb 13;103(6):904-12 - PubMed
  12. Biopharm Drug Dispos. 2005 Jul;26(5):205-10 - PubMed
  13. Eur J Clin Pharmacol. 2003 Aug;59(4):291-5 - PubMed
  14. J Biochem Biophys Methods. 2002 Dec 31;54(1-3):357-68 - PubMed
  15. Am J Kidney Dis. 1993 Nov;22(5):745-54 - PubMed
  16. Am J Hypertens. 1992 Dec;5(12 Pt 2):221S-235S - PubMed
  17. J Chromatogr B Biomed Sci Appl. 1999 Nov 12;734(2):325-30 - PubMed
  18. J Cardiovasc Pharmacol. 1988;12 Suppl 7:S22-6 - PubMed
  19. Cardiovasc Drugs Ther. 1997 Jan;10 Suppl 3:869-72 - PubMed
  20. Clin Pharmacokinet. 2005;44(8):797-814 - PubMed
  21. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Sep 15;873(1):77-85 - PubMed

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