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Yao CS, Waterfield JD, Shen Y, et al. In vitro antibacterial effect of carbamide peroxide on oral biofilm. J Oral Microbiol. 2013;5doi: 10.3402/jom.v5i0.20392.
Yao, C. S., Waterfield, J. D., Shen, Y., Haapasalo, M., & Macentee, M. I. (2013). In vitro antibacterial effect of carbamide peroxide on oral biofilm. Journal of oral microbiology, 5. https://doi.org/10.3402/jom.v5i0.20392
Yao, Chao Shu, et al. "In vitro antibacterial effect of carbamide peroxide on oral biofilm." Journal of oral microbiology vol. 5 (2013). doi: https://doi.org/10.3402/jom.v5i0.20392
Yao CS, Waterfield JD, Shen Y, Haapasalo M, Macentee MI. In vitro antibacterial effect of carbamide peroxide on oral biofilm. J Oral Microbiol. 2013 Jun 12;5. doi: 10.3402/jom.v5i0.20392. Print 2013. PMID: 23785567; PMCID: PMC3682087.
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J Oral Microbiol. 2013 Jun 12;5. doi: 10.3402/jom.v5i0.20392. Print 2013.

In vitro antibacterial effect of carbamide peroxide on oral biofilm.

Journal of oral microbiology

Chao Shu Yao, John Douglas Waterfield, Ya Shen, Markus Haapasalo, Michael I Macentee

Affiliations

  1. University of British Columbia, Vancouver, BC, Canada.

PMID: 23785567 PMCID: PMC3682087 DOI: 10.3402/jom.v5i0.20392

Abstract

This study compared the effects of carbamide peroxide (CP) and chlorhexidine (CHX) on oral biofilm in vitro. Collagen-coated hydroxyapatite discs were inoculated with subgingival plaque. After 3 weeks, the emergent biofilms were subjected to 1-, 3-, and 10-min exposures of a 1% CHX gel, a 5% CP gel and rinse, and a 10% CP gel and rinse. Subsequently, the biofilms were stained using a two-colour fluorescent dye kit for confocal laser scanning microscopy, and the volume ratio of dead bacteria to all bacteria was analysed. Compared to a non-treated gel control, the active agents killed bacteria on all the discs, with higher concentration and longer exposure times killing more bacteria. The rinse form disrupted the biofilm quicker than the gel form. Overall, 10% CP showed more disruption of biofilm and a greater proportion of killed bacteria than 1% CHX (p<0.05).

Keywords: antibacterial; biofilm; carbamide peroxide; confocal laser scanning microscopy

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