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Schaumann R, Knoop N, Genzel GH, et al. Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry. Open Microbiol J. 2013;7:118-22doi: 10.2174/1874285801307010118.
Schaumann, R., Knoop, N., Genzel, G. H., Losensky, K., Rosenkranz, C., Stîngu, C. S., Schellenberger, W., Rodloff, A. C., & Eschrich, K. (2013). Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry. The open microbiology journal, 7118-22. https://doi.org/10.2174/1874285801307010118
Schaumann, Reiner, et al. "Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry." The open microbiology journal vol. 7 (2013): 118-22. doi: https://doi.org/10.2174/1874285801307010118
Schaumann R, Knoop N, Genzel GH, Losensky K, Rosenkranz C, Stîngu CS, Schellenberger W, Rodloff AC, Eschrich K. Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry. Open Microbiol J. 2013 Jun 28;7:118-22. doi: 10.2174/1874285801307010118. Print 2013. PMID: 23919091; PMCID: PMC3722536.
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Open Microbiol J. 2013 Jun 28;7:118-22. doi: 10.2174/1874285801307010118. Print 2013.

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry.

The open microbiology journal

Reiner Schaumann, Nicolas Knoop, Gelimer H Genzel, Kevin Losensky, Christiane Rosenkranz, Catalina S Stîngu, Wolfgang Schellenberger, Arne C Rodloff, Klaus Eschrich

Affiliations

  1. Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Leipzig, Germany.

PMID: 23919091 PMCID: PMC3722536 DOI: 10.2174/1874285801307010118

Abstract

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.

Keywords: E. coli; EHEC; EPEC; Enterobacteriaceae; MALDI-TOF MS; Salmonella spp; Shigella sonnei.

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