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Lachmann N, Todorova K, Schulze H, et al. Luminex(®) and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion. Transfus Med Hemother. 2013;40(3):182-9doi: 10.1159/000351459.
Lachmann, N., Todorova, K., Schulze, H., & Schönemann, C. (2013). Luminex(®) and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion. Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 40(3), 182-9. https://doi.org/10.1159/000351459
Lachmann, Nils, et al. "Luminex(®) and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion." Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie vol. 40,3 (2013): 182-9. doi: https://doi.org/10.1159/000351459
Lachmann N, Todorova K, Schulze H, Schönemann C. Luminex(®) and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion. Transfus Med Hemother. 2013 Jun;40(3):182-9. doi: 10.1159/000351459. Epub 2013 May 08. PMID: 23922543; PMCID: PMC3725018.
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Transfus Med Hemother. 2013 Jun;40(3):182-9. doi: 10.1159/000351459. Epub 2013 May 08.

Luminex(®) and its applications for solid organ transplantation, hematopoietic stem cell transplantation, and transfusion.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Nils Lachmann, Kremena Todorova, Harald Schulze, Constanze Schönemann

Affiliations

  1. Center for Tumor Medicine, Charité-Universitätsmedizin Berlin - Campus Virchow-Klinikum, Berlin, Germany ; ZTB Zentrum für Transfusionsmedizin und Zelltherapie Berlin, HLA Tissue Typing Laboratory, Berlin, Germany.

PMID: 23922543 PMCID: PMC3725018 DOI: 10.1159/000351459

Abstract

SUMMARY: The detection of antibodies against the human leukocyte antigen (HLA) complex has become indispensable in every clinical practice. The development of solid-phase assays like the Luminex allows the standardized measurement of anti-HLA antibodies (HLAab) with high sensitivity, albeit the relevance for some clinical settings remains a matter of debate. In this review we aim to describe the principle of Luminex-based antibody detection, including two modifications that allow identifying solely complement-activating antibodies. We then describe three applications for Luminex: i) detection of HLAab preceding solid-organ transplantation and monitoring of donor-specific antibodies posttransplant as a risk factor for antibody-mediated rejection; ii) presence of HLAab in recipients as a risk for graft failure in hematopoietic stem cell transplantation, especially in haploidentical or mismatched transplantations; iii) role of HLAab in blood transfusion including refractory thrombocytopenia and selection of suitable platelet donors, transfusion-related lung injury after plasma transfusion, and immunization against HLA after red blood cell transfusion despite leukodepletion. Although the Luminex platform constitutes a potent technology for HLA antibody detection, some drawbacks require the well-educated analysis and interpretation of data in critical cases. In addition, Luminex has become an important tool to identify clinically relevant antibodies.

Keywords: HLA antibodies; Luminex; Transfusion

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