Display options
Share it on

Food Environ Virol. 2013 Jul 30; doi: 10.1007/s12560-013-9119-z. Epub 2013 Jul 30.

Rotavirus Occurrence in Shellfish with Low Levels of E. coli.

Food and environmental virology

Francesco Bagordo, Tiziana Grassi, Adele Idolo, Francesca Serio, Giovanni Gabutti, Antonella De Donno

Affiliations

  1. Laboratory of Hygiene, Department of Biological and Environmental Sciences and Technologies (Di.S.Te.B.A.), University of Salento, Via Prov.le Lecce-Monteroni, Lecce, 73100, Italy.

PMID: 23897404 DOI: 10.1007/s12560-013-9119-z

Abstract

The purpose of this study was to evaluate "in field" the accumulation of virus in shellfish and compare it with the concentration of bacterial indicators. Individuals of Mytilus galloprovincialis were placed in two sampling station located in a contaminated coastal bay and in one control station located one kilometer offshore. The presence of Rotavirus and E. coli was assessed weekly both in seawater and in shellfish samples. The Rotavirus genome was detected in water, preliminarily concentrated by tangential flow ultrafiltration method, and in hepatopancreas of mussels by Real-Time PCR. E. coli was enumerated in water matrices by a filtering method and in mussels by the MPN method. Rotaviruses were not recorded in seawater, while in mussels they were detected since third week after placement. E. coli in mussels were always below the limits set in the Regulation (EC) 854/2004. This study suggests the need for a viral indicator to insure the safety for consumption of shellfish.

References

  1. Food Environ Virol. 2013 Jun;5(2):110-8 - PubMed
  2. Int J Food Microbiol. 2002 May 5;75(1-2):11-8 - PubMed
  3. Environ Monit Assess. 2010 May;164(1-4):199-205 - PubMed
  4. Appl Environ Microbiol. 2005 Mar;71(3):1453-61 - PubMed
  5. JAMA. 2001 Jan 24-31;285(4):405-6 - PubMed
  6. Clin Microbiol Rev. 1994 Oct;7(4):419-25 - PubMed
  7. Food Environ Virol. 2012 Jun;4(2):81-8 - PubMed
  8. Microbiol Mol Biol Rev. 2005 Jun;69(2):357-71 - PubMed
  9. J Virol Methods. 2000 Oct;90(1):1-14 - PubMed
  10. BMC Infect Dis. 2009 Apr 15;9:43 - PubMed
  11. J Virol Methods. 2012 Dec;186(1-2):198-202 - PubMed
  12. Appl Environ Microbiol. 2002 Oct;68(10):5167-9 - PubMed
  13. J Appl Microbiol. 2000 Feb;88(2):293-8 - PubMed
  14. Appl Environ Microbiol. 2005 Oct;71(10):6049-53 - PubMed
  15. Appl Environ Microbiol. 2001 Jan;67(1):179-84 - PubMed
  16. Int J Food Microbiol. 2007 Mar 10;114(2):252-7 - PubMed
  17. J Virol Methods. 2004 Feb;115(2):177-82 - PubMed
  18. Food Environ Virol. 2012 Jun;4(2):73-80 - PubMed
  19. Commun Dis Public Health. 1998 Sep;1(3):188-96 - PubMed
  20. J Clin Microbiol. 2007 Mar;45(3):1011-5 - PubMed
  21. Appl Environ Microbiol. 2000 Aug;66(8):3241-8 - PubMed
  22. FEMS Microbiol Rev. 2012 Jul;36(4):786-814 - PubMed
  23. J Appl Microbiol. 2010 Dec;109(6):1979-87 - PubMed
  24. FEMS Microbiol Rev. 2004 Oct;28(4):441-53 - PubMed
  25. Ann Ig. 2004 Jan-Apr;16(1-2):281-9 - PubMed
  26. Int J Food Microbiol. 1999 Aug 1;49(1-2):43-8 - PubMed
  27. Water Res. 2008 Jun;42(12):3033-46 - PubMed
  28. New Microbiol. 2007 Oct;30(4):471-5 - PubMed
  29. Emerg Infect Dis. 2006 Feb;12(2):304-6 - PubMed
  30. Water Sci Technol. 2006;54(11-12):301-8 - PubMed
  31. Vaccine. 2009 May 26;27(25-26):3450-3 - PubMed
  32. Int J Food Microbiol. 2007 Feb 15;113(3):296-302 - PubMed
  33. Int J Food Microbiol. 2000 Jul 25;59(1-2):81-116 - PubMed
  34. Water Res. 2008 May;42(10-11):2618-28 - PubMed

Publication Types