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Ganaie JA, Gautam V, Shrivastava VK. Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus. J Reprod Infertil. 2011;12(1):3-7
Ganaie, J. A., Gautam, V., & Shrivastava, V. K. (2011). Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus. Journal of reproduction & infertility, 12(1), 3-7.
Ganaie, Javid Ahmad, et al. "Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus." Journal of reproduction & infertility vol. 12,1 (2011): 3-7.
Ganaie JA, Gautam V, Shrivastava VK. Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus. J Reprod Infertil. 2011 Jan;12(1):3-7. PMID: 23926493; PMCID: PMC3719281.
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J Reprod Infertil. 2011 Jan;12(1):3-7.

Effects of Kamdhenu Ark and Active Immunization by Gonadotropin Releasing Hormone Conjugate (GnRH-BSA) on Gonadosomatic Indices (GSI) and Sperm Parameters in Male Mus musculus.

Journal of reproduction & infertility

Javid Ahmad Ganaie, Varsha Gautam, Vinoy Kumar Shrivastava

Affiliations

  1. Endocrinology Laboratory, Department of Biosciences, Barkatullah University, Bhopal, India.

PMID: 23926493 PMCID: PMC3719281

Abstract

BACKGROUND: Active immunization against GnRH decreases the secretion of gonadotropins and causes cessation of gonadal function, thereby, inducing infertility. Based on the immunoenhancing activity of Kamdhenu ark (distilled cow urine), this study was performed to evaluate its effects on the gonadosomatic indices (GSI) and sperm parameters in male mice receiving a GnRH contraceptive vaccine.

METHODS: Sixty adult male mice of Parke's strain were divided into three groups of twenty. Group I served as the controls, while group II was immunized by GnRH-BSA conjugate (50/0.2/35 µg/ml/g BW) by four intraperitoneal injections at different intervals on days 1, 30, 60 and 90. However, group III was supplemented daily by oral Kamdhenu ark (100 ppm) along with GnRH-BSA immunizations. The animals were sacrificed after 30, 60, 90 and 120 days and their testis and epididymis were dissected out weighed and semen analysis was performed.

RESULTS: GSI values, sperm motility, sperm count and sperm morphology in male Mus musculus were decreased significantly in all the experimental groups as compared to the control group (p<0.01). Kamdhenu ark significantly enhanced the effect of GnRH vaccine on the aforesaid parameters especially in 90 and 120 days treated groups (p<0.05).

CONCLUSION: The changes witnessed in sperm parameters suggested that the GnRH-BSA immunization suppressed the activities of gonadotropins and testosterone directly through hypothalamo-hypophysial-gonadal axis and indirectly by acting on the testes which may modulate the sperm morphology, sperm count and motility. However, Kamdhenu ark seems to have enhanced these effects because of its immune-modulatory properties too.

Keywords: GnRH-BSA; Gonadosomatic indices (GSI); Immunization; Kamdhenu ark; Mus musculus; Sperm parameters

References

  1. Fertil Steril. 1972 Mar;23(3):186-90 - PubMed
  2. Biol Reprod. 1994 Feb;50(2):271-80 - PubMed
  3. J Reprod Immunol. 2004 Dec;64(1-2):107-19 - PubMed
  4. Theriogenology. 2004 Sep 15;62(6):1116-30 - PubMed
  5. J Reprod Fertil Suppl. 1991;44:183-90 - PubMed
  6. Br J Cancer. 2000 Aug;83(4):443-6 - PubMed
  7. J Anim Sci. 2000 Nov;78(11):2778-83 - PubMed
  8. Aust J Biotechnol. 1990 Jul;4(3):166-70, 176 - PubMed
  9. J Anim Sci. 1988 Mar;66(3):719-26 - PubMed
  10. Theriogenology. 1987 Apr;27(4):571-80 - PubMed
  11. J Anim Sci. 1995 Oct;73(10):3030-7 - PubMed
  12. Biol Reprod. 1985 Oct;33(3):569-77 - PubMed
  13. Endocrinology. 1990 Feb;126(2):992-100 - PubMed
  14. Am J Reprod Immunol. 2000 Nov;44(5):266-74 - PubMed
  15. Vaccine. 1994 Jun;12(8):741-6 - PubMed
  16. Toxicol Sci. 2000 Jan;53(1):92-9 - PubMed
  17. Anim Reprod Sci. 2009 Oct;115(1-4):88-102 - PubMed
  18. Int J Fertil. 1991 Jan-Feb;36(1):43-7 - PubMed

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