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O'Daly JA, Spinetti HM, Gleason J, et al. Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments. J Parasitol Res. 2013;2013:657016doi: 10.1155/2013/657016.
O'Daly, J. A., Spinetti, H. M., Gleason, J., & Rodríguez, M. B. (2013). Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments. Journal of parasitology research, 2013657016. https://doi.org/10.1155/2013/657016
O'Daly, José A, et al. "Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments." Journal of parasitology research vol. 2013 (2013): 657016. doi: https://doi.org/10.1155/2013/657016
O'Daly JA, Spinetti HM, Gleason J, Rodríguez MB. Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments. J Parasitol Res. 2013;2013:657016. doi: 10.1155/2013/657016. Epub 2013 Jun 13. PMID: 23844278; PMCID: PMC3697410.
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J Parasitol Res. 2013;2013:657016. doi: 10.1155/2013/657016. Epub 2013 Jun 13.

Clinical and Immunological Analysis of Cutaneous Leishmaniasis before and after Different Treatments.

Journal of parasitology research

José A O'Daly, Humberto M Spinetti, Joe Gleason, María B Rodríguez

Affiliations

  1. Astralis LTD, 1076 Stuyvesant Avenue, Irvington, NJ 07111, USA ; Instituto Venezolano de Investigaciones Científicas (IVIC), Altos de Pipe, Km 10 Carretera Panamericana, Caracas 1010A, Venezuela.

PMID: 23844278 PMCID: PMC3697410 DOI: 10.1155/2013/657016

Abstract

Amastigotes from L. (L.)amazonensis (La), L. (L.)venezuelensis (Lv), L. (V.)brasiliensis (Lb), and L. (L.)chagasi (Lch) were cultured in a free cells liquid culture medium. Patients (n = 87) from a cutaneous leishmaniasis (CL) hyperendemic region receiving different treatments were followed up from January 1994 to August 2000. Time for remission of lesions were spontaneous remission (SR) 7 weeks; Glucantime (Glu) chemotherapy 9 weeks; immunotherapy with La, Lv, Lb, and Lch amastigotes Tosyl-Lysil Chloromethyl-ketone (TLCK) treated and Nonidet P-40(NP-40) extracted (VT) 7 weeks. Delayed type hypersensitivity (DTH) response with leishmanine intradermic reaction (IDR) was higher in CL patients than healthy controls (P < 0.05) and increased in active secondary versus primary infection (P < 0.001) with diagnostic value 1.74 for active infection and 1.81 postclinical remission. Antibodies to amastigotes characterized by Enzyme Linked Immunosorbent Assay (ELISA) decreased in sera postclinical remission versus active infections (P < 0.001), with a diagnostic value from 1.50 to 1.84. Immunoblottings antigenic bands frequency as well as Integral Optical Density (IOD) Area Densitometry decreased with sera from SR, after Glu or VT treatments in CL volunteers. Intracellular parasitism is due to normal antibodies recognizing parasite antigens after inoculation by vector. VT vaccine induced mainly cellular immunity, for remission of lesions and protection from CL infection.

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